HIST1H2AJ antibody (AA 1-128)

Catalog No. ABIN1859162

The Rabbit Polyclonal anti-HIST1H2AJ antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect HIST1H2AJ in samples from Human.

Quick Overview for HIST1H2AJ antibody (AA 1-128) (ABIN1859162)

Target: HIST1H2AJ (Histone Cluster 1, H2aj (HIST1H2AJ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H2AJ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-HIST1H2AJ Antibody

Binding Specificity: AA 1-128

Purpose: Polyclonal Antibody to Histone Cluster 1, H2aj (HIST1H2AJ)

Specificity: The antibody is a rabbit polyclonal antibody raised against HIST1H2AJ. It has been selected for its ability to recognize HIST1H2AJ in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Histone Cluster 1, H2aj (HIST1H2AJ) corresdonding to Met1~Lys128 (Accession # Q99878) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunocytochemistry in formalin fixed cells: 5-20 μg/mLImmunohistochemistry in formalin fixed frozen section: 5-20 μg/mLImmunohistochemistry in paraffin section: 5-20 μg/mLEnzyme-linked Immunosorbent Assay: 0.05-2 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HIST1H2AJ

Target: HIST1H2AJ (Histone Cluster 1, H2aj (HIST1H2AJ))

Alternative Name: HIST1H2AJ

Background: H2AFE, H2A/E, H2A Histone Family Member E

Pathways: Telomere Maintenance