Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1) antibody

Antigen: Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1)

Synonyms: APR, NOXA, Noxa, PMAIP1, zgc:162379

Clonality: Monoclonal (114C307)

Host: Rat

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN187551

Additional Information

Alternative name: NOXA

Immunogen: Recombinant human Noxa.

Isotype: IgG1

Clone: 114C307

Specificity: Recognizes human Noxa. Detects a band of approximately 11 kDa by Western blot. Species Reactivity: Human. Others not tested.

Application Details

Application Notes: Western blot: Use at 1-2 µg/mL. Immunocytochemistry The optimal dilution for a specific application should be determined by the researcher.

Buffer: 100 µg of liquid Protein G affinity-purified monoclonal antibody at 0.5 mg/mL in PBS containing protein stabilizer and 0.05% sodium azide.

Storage: Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Images

anti-Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1) antibody

Publications

Smit, Hallaert, Spijker et al.: "Differential Noxa/Mcl-1 balance in peripheral versus lymph node chronic lymphocytic leukemia cells correlates with survival capacity." in: Blood, Vol. 109, Issue 4, pp. 1660-8, 2007 (PubMed).

Guomez-Benito, Balsas, Carvajal-Vergara et al.: "Mechanism of apoptosis induced by IFN-alpha in human myeloma cells: role of Jak1 and Bim and potentiation by rapamycin." in: Cellular signalling, Vol. 19, Issue 4, pp. 844-54, 2007 (PubMed).

Puerez-Galuan, Rouue, Villamor et al.: "The BH3-mimetic GX15-070 synergizes with bortezomib in mantle cell lymphoma by enhancing Noxa-mediated activation of Bak." in: Blood, Vol. 109, Issue 10, pp. 4441-9, 2007 (PubMed).

Kojima, Konopleva, Samudio et al.: "Mitogen-activated protein kinase kinase inhibition enhances nuclear proapoptotic function of p53 in acute myelogenous leukemia cells." in: Cancer research, Vol. 67, Issue 7, pp. 3210-9, 2007 (PubMed).

Kurata, Yanagisawa, Ohira et al.: "Stress via p53 pathway causes apoptosis by mitochondrial Noxa upregulation in doxorubicin-treated neuroblastoma cells." in: Oncogene, Vol. 27, Issue 6, pp. 741-54, 2008 (PubMed).

Anderton, Yee, Smith et al.: "Two Epstein-Barr virus (EBV) oncoproteins cooperate to repress expression of the proapoptotic tumour-suppressor Bim: clues to the pathogenesis of Burkitt's lymphoma." in: Oncogene, Vol. 27, Issue 4, pp. 421-33, 2008 (PubMed).

Poulaki, Mitsiades, Kotoula et al.: "The proteasome inhibitor bortezomib induces apoptosis in human retinoblastoma cell lines in vitro." in: Investigative ophthalmology & visual science, Vol. 48, Issue 10, pp. 4706-19, 2007 (PubMed).

Nefedova, Sullivan, Bolick et al.: "Inhibition of Notch signaling induces apoptosis of myeloma cells and enhances sensitivity to chemotherapy." in: Blood, Vol. 111, Issue 4, pp. 2220-9, 2008 (PubMed).

Flanagan, Letai: "BH3 domains define selective inhibitory interactions with BHRF-1 and KSHV BCL-2." in: Cell death and differentiation, Vol. 15, Issue 3, pp. 580-8, 2008 (PubMed).

Deng, Shimamura, Perera et al.: "Proapoptotic BH3-only BCL-2 family protein BIM connects death signaling from epidermal growth factor receptor inhibition to the mitochondrion." in: Cancer research, Vol. 67, Issue 24, pp. 11867-75, 2007 (PubMed).

Sasaki, Negishi, Idogawa et al.: "Histone deacetylase inhibitor FK228 enhances adenovirus-mediated p53 family gene therapy in cancer models." in: Molecular cancer therapeutics, Vol. 7, Issue 4, pp. 779-87, 2008 (PubMed).

Kojima, Shimanuki, Shikami et al.: "The dual PI3 kinase/mTOR inhibitor PI-103 prevents p53 induction by Mdm2 inhibition but enhances p53-mediated mitochondrial apoptosis in p53 wild-type AML." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 22, Issue 9, pp. 1728-36, 2008 (PubMed).

Hallaert, Jaspers, van Noesel et al.: "c-Abl kinase inhibitors overcome CD40-mediated drug resistance in CLL: implications for therapeutic targeting of chemoresistant niches." in: Blood, Vol. 112, Issue 13, pp. 5141-9, 2008 (PubMed).

Braun, Zhang, Savelyeva et al.: "p53-Responsive micrornas 192 and 215 are capable of inducing cell cycle arrest." in: Cancer research, Vol. 68, Issue 24, pp. 10094-104, 2008 (PubMed).

van Gent, Kater, Otto et al.: "In vivo dynamics of stable chronic lymphocytic leukemia inversely correlate with somatic hypermutation levels and suggest no major leukemic turnover in bone marrow." in: Cancer research, Vol. 68, Issue 24, pp. 10137-44, 2008 (PubMed).

Stankiewicz, Livingstone, Mohseni et al.: "Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70." in: Cell death and differentiation, Vol. 16, Issue 4, pp. 638-47, 2009 (PubMed).

Hauck, Chao, Litz et al.: "Alterations in the Noxa/Mcl-1 axis determine sensitivity of small cell lung cancer to the BH3 mimetic ABT-737." in: Molecular cancer therapeutics, Vol. 8, Issue 4, pp. 883-92, 2009 (PubMed).

Ploner, Rainer, Lobenwein et al.: "Repression of the BH3-only molecule PMAIP1/Noxa impairs glucocorticoid sensitivity of acute lymphoblastic leukemia cells." in: Apoptosis : an international journal on programmed cell death, Vol. 14, Issue 6, pp. 821-8, 2009 (PubMed).

Forest, Hadjeres, Bertrand et al.: "Optimisation and molecular signalling of apoptosis in sequential cryotherapy and chemotherapy combination in human A549 lung cancer xenografts in SCID mice." in: British journal of cancer, Vol. 100, Issue 12, pp. 1896-902, 2009 (PubMed).

Kutuk, Arisan, Tezil et al.: "Cisplatin overcomes Bcl-2-mediated resistance to apoptosis via preferential engagement of Bak: critical role of Noxa-mediated lipid peroxidation." in: Carcinogenesis, 2009 (PubMed).