TLR3 antibody

Catalog No. ABIN187555

Product Details anti-TLR3 Antibody

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes human Toll-like Receptor 3 (TLR3). Species Reactivity: Human and mouse. Others not tested.

Immunogen: Synthetic peptides derived from the cytoplasmic portion of human TLR3.

Clone: 40C1285-6

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Use 2-5 µg/106 cells for intracellular staining. Immunoprecipitation: Use 2 µg/106 transfected cells. Western blot: Use at 1-3 µg/mL. Immunohistochemistry: (paraffin sections) Immunocytochemistry The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 µg of liquid Protein G purified monoclonal antibody at 0.5 mg/mL in PBS containing protein stabilizer and 0.05% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for TLR3

Target: TLR3 (Toll-Like Receptor 3 (TLR3))

Alternative Name: CD283 / TLR3 (TLR3 Products)

Background: The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. TLR3 cDNA codes for a protein, ~120 kDa. TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes. The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades