ATG4B antibody (C-Term)

Catalog No. ABIN1882065

This anti-ATG4B antibody is a Rabbit Polyclonal antibody detecting ATG4B in WB, IHC (p) and IF. Suitable for Human. This Primary Antibody has been cited in 6+ publications.

Quick Overview for ATG4B antibody (C-Term) (ABIN1882065)

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: RB7560

Product Details anti-ATG4B Antibody

Binding Specificity: AA 358-390, C-Term

Predicted Reactivity: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 358-390 amino acids from the C-terminal region of human ATG4B.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ATG4B antibody (ABIN1882065)

Li, Chen, Ye, Vogt, Yin: "A high-throughput FRET-based assay for determination of Atg4 activity." in: Autophagy, Vol. 8, Issue 3, pp. 401-12, (2012) (PubMed).

Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, (2011) (PubMed).

Li, Hou, Wang, Chen, Shao, Yin: "Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates." in: The Journal of biological chemistry, Vol. 286, Issue 9, pp. 7327-38, (2011) (PubMed).

Geng, Kohli, Klocke, Roth: "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent." in: Neuro-oncology, Vol. 12, Issue 5, pp. 473-81, (2010) (PubMed).

Pontén, Westermark: "Properties of human malignant glioma cells in vitro." in: Medical biology, Vol. 56, Issue 4, pp. 184-93, (1978) (PubMed).

Westermark, Pontén, Hugosson: "Determinants for the establishment of permanent tissue culture lines from human gliomas." in: Acta pathologica et microbiologica Scandinavica. Section A, Pathology, Vol. 81, Issue 6, pp. 791-805, (1974) (PubMed).

Target Details for ATG4B

Target: ATG4B (Autophagy related 4B Cysteine Peptidase (ATG4B))

Alternative Name: ATG4B

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Molecular Weight: 44294

NCBI Accession: NP_037457, NP_847896

UniProt: Q9Y4P1

Pathways: Autophagy