The Rabbit Polyclonal anti-POLR2J3 antibody has been validated for WB. It is suitable to detect POLR2J3 in samples from Human. There are 2+ publications available.
Quick Overview for POLR2J3 antibody (N-Term) (ABIN1882184)
Target
POLR2J3
(Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Clone
RB45111
Binding Specificity
AA 1-25, N-Term
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This POLR2J3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-25 amino acids from the N-terminal region of human POLR2J3.
Isotype
Ig Fraction
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Grandemange, Schaller, Yamano, Du Manoir, Shpakovski, Mattei, Kedinger, Vigneron: "A human RNA polymerase II subunit is encoded by a recently generated multigene family." in: BMC molecular biology, Vol. 2, pp. 14, (2003) (PubMed).
Hillier, Fulton, Fulton, Graves, Pepin, Wagner-McPherson, Layman, Maas, Jaeger, Walker, Wylie, Sekhon, Becker, OLaughlin, Schaller, Fewell, Delehaunty, Miner, Nash, Cordes, Du, Sun, Edwards et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, (2003) (PubMed).
Target
POLR2J3
(Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
Alternative Name
POLR2J3
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft (By similarity).