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Ezrin antibody (Tyr353)

The Rabbit Polyclonal anti-Ezrin antibody has been validated for WB. It is suitable to detect Ezrin in samples from Human and Mouse.
Catalog No. ABIN197258

Quick Overview for Ezrin antibody (Tyr353) (ABIN197258)

Target

See all Ezrin (EZR) Antibodies
Ezrin (EZR)

Reactivity

  • 156
  • 80
  • 73
  • 8
  • 8
  • 7
  • 5
  • 4
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
Human, Mouse

Host

  • 136
  • 20
  • 3
  • 2
Rabbit

Clonality

  • 128
  • 32
Polyclonal

Conjugate

  • 99
  • 7
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Ezrin antibody is un-conjugated

Application

  • 133
  • 59
  • 44
  • 18
  • 15
  • 15
  • 13
  • 13
  • 13
  • 11
  • 10
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Binding Specificity

    • 28
    • 16
    • 9
    • 9
    • 9
    • 9
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Tyr353

    Specificity

    Ezrin antibody detects endogenous levels of total Ezrin protein.

    Purification

    Affinity-chromatography using epitope-specific immunogen

    Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
  • Application Notes

    Western Blot: 1: 500approx. 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    -20 °C
  • Target

    Ezrin (EZR)

    Alternative Name

    Ezrin

    Background

    Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81

    Gene ID

    7430

    NCBI Accession

    NP_001104547

    UniProt

    P15311

    Pathways

    Maintenance of Protein Location
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