Ezrin antibody (Tyr353)
Quick Overview for Ezrin antibody (Tyr353) (ABIN197258)
Target
See all Ezrin (EZR) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- Tyr353
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Specificity
- Ezrin antibody detects endogenous levels of total Ezrin protein.
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Purification
- Affinity-chromatography using epitope-specific immunogen
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Immunogen
- The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
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Application Notes
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Western Blot: 1: 500approx. 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- -20 °C
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- Ezrin (EZR)
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Alternative Name
- Ezrin
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Background
- Ezrin (also known as p81, 80K, or cytovillin) is a cytoplasmic protein which is enriched in microvilli and other cell surface structures. Its NH2-terminal domain is associated with the plasma membrane and the COOH-terminal domain with the cytoskeleton. Ezrin has an actin-binding capability. Ezrin is expressed in epithelial cells but not in mesenchymal cells. Ezrin is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas. Ezrin serves as a tyrosine kinase substrate, and is phosphorylated in EGF-stimulated cells.Synonyms: Cytovillin, EZR, VIL2, Villin-2, p81
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Gene ID
- 7430
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NCBI Accession
- NP_001104547
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UniProt
- P15311
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Pathways
- Maintenance of Protein Location
Target
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