IL17D antibody (AA 18-202)

Catalog No. ABIN204351

Product Details anti-IL17D Antibody

Target: IL17D (Interleukin 17D (IL17D))

Binding Specificity: AA 18-202

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL17D antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes human IL-27. Does not cross-react with rhIL-17, rhIL17B, rhIL-17C, rhIL-17E, or rhIL-17F. Species sequence homology: Mouse: 86 % .

Purification: Protein G purified

Immunogen: E. coli-derived recombinant human Interleukin 27 (aa18-202).

Clone: 246018

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-P, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and ELISA. Western Blot: 1-2 μg/mL. Detection limit is approximately 25 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Add 10 μL of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 200 μL. Incubate for 30 minutes and then wash cells with 0.1 % saponin prior to addition of a secondary developing reagent. Cells should be washed a final time in 0.1 % saponin prior to flow cytometric analysis. Direct ELISA: 0.5-1 μg/mL. Detection limit is ~5 ng/well. Immunohistochemistry: 8-25 μg/mL with appropriate secondary reagents to detect IL-17D in paraffin-embedded normal heart tissue sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile 40-50 % glycerol, PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Storage: -20 °C

Storage Comment: Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.

Target Details for IL17D

Target: IL17D (Interleukin 17D (IL17D))

Alternative Name: IL17D (IL17D Products)

Background: Name/Gene ID: IL17D
Family: Interleukin

Synonyms: IL17D, IL-27, Interleukin 27, Interleukin-17D, Interleukin-27, IL-17D, IL-22, Interleukin 17D

Gene ID: 53342

UniProt: Q8TAD2

Pathways: Cellular Response to Molecule of Bacterial Origin