ZAP70 antibody (pTyr493)

Catalog No. ABIN218908

Product Details anti-ZAP70 Antibody

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Binding Specificity: pTyr493

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP)

Specificity: Detects endogenous levels of Tyr493 phosphorylated human Zap-70 (Mr 70kD). Species cross-reactivity: Predicted to cross-react with mouse based on sequence homology.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr493 of human Zap-70 (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:100), IP (1:100), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. Western Blot: 1:1000. Immunoprecipitation: 1:100. Flow Cytometry: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70 (ZAP70 Products)

Background: Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase

Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway