ZAP70 antibody

Catalog No. ABIN969464

Product Details anti-ZAP70 Antibody

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: purified

Immunogen: Purified recombinant fragment of human ZAP70 expressed in E. coli.

Clone: 3D2A4

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

References for anti-ZAP70 antibody (ABIN969464)

Gelkop, Gish, Babichev, Pawson, Isakov: "T cell activation-induced CrkII binding to the Zap70 protein tyrosine kinase is mediated by Lck-dependent phosphorylation of Zap70 tyrosine 315." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 12, pp. 8123-32, (2005) (PubMed).

Carreras, Villamor, Colomo, Moreno, Ramón y Cajal, Crespo, Tort, Bosch, López-Guillermo, Colomer, Montserrat, Campo: "Immunohistochemical analysis of ZAP-70 expression in B-cell lymphoid neoplasms." in: The Journal of pathology, Vol. 205, Issue 4, pp. 507-13, (2005) (PubMed).

Hivroz: "[Everything you ever wanted to know about ZAP-70]." in: Médecine sciences : M/S, Vol. 21, Issue 2, pp. 150-5, (2005) (PubMed).

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70 (ZAP70 Products)

Synonyms: SRK antibody, STCD antibody, STD antibody, TZK antibody, ZAP-70 antibody, 70kDa antibody, AI327364 antibody, Srk antibody, mrtle antibody, mur antibody, zap70 antibody, MGC80670 antibody, MGC88962 antibody, ZAP70 antibody, zgc:110383 antibody, zeta chain of T-cell receptor associated protein kinase 70 antibody, putative tyrosine-protein kinase zap-70 antibody, zeta-chain (TCR) associated protein kinase antibody, zeta chain of T cell receptor associated protein kinase 70 antibody, zeta-chain (TCR) associated protein kinase 70kDa antibody, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antibody, zeta chain of T cell receptor associated protein kinase 70kDa antibody, tyrosine-protein kinase ZAP-70 antibody, ZAP70 antibody, Smp_149470 antibody, Zap70 antibody, zap70.L antibody, zap70 antibody, LOC707555 antibody, LOC100342021 antibody, LOC100390922 antibody, LOC100542877 antibody

Background:

Description: ZAP70 (zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. ZAP70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the ZAP70 gene. ZAP70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.

Aliases: SRK, STD, TZK

Molecular Weight: 70 kDa

Gene ID: 7535

HGNC: 7535

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway