ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (C-Term)

Details for Product anti-ZAP70 Antibody No. ABIN125756, Supplier: Log in to see
Antigen
  • zap70
  • MGC80670
  • MGC88962
  • ZAP70
  • 70kDa
  • AI327364
  • Srk
  • TZK
  • ZAP-70
  • mrtle
  • mur
  • SRK
  • STCD
  • STD
  • zeta chain of T cell receptor associated protein kinase 70kDa L homeolog
  • zeta chain of T cell receptor associated protein kinase 70kDa
  • zeta chain of T cell receptor associated protein kinase 70
  • tyrosine-protein kinase ZAP-70
  • putative tyrosine-protein kinase zap-70
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain (TCR) associated protein kinase
  • zeta chain of T-cell receptor associated protein kinase 70
  • zap70.L
  • zap70
  • ZAP70
  • LOC707555
  • Smp_149470
  • LOC100342021
  • LOC100390922
  • LOC100542877
  • Zap70
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
C-Term
120
53
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36
30
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25
14
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10
7
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2
1
1
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1
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Reactivity
Human
683
246
152
14
11
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8
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1
Host
Mouse
346
319
17
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This ZAP70 antibody is un-conjugated
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29
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1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
356
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163
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65
52
50
26
8
7
4
4
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Options
Supplier
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Supplier Product No.
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Immunogen Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag
Clone ZAP-03
Isotype IgG1
Specificity The antibody ZAP-03 reacts with ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.
ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Plasmids, Primers & others Plasmids, Primers & others ZAP70 products on genomics-online (e.g. as negative or positive controls)
Antigen zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)
Alternative Name ZAP70 (ZAP70 Antibody Abstract)
Background The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
Pathways TCR Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody (ABIN125756) Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell le...
Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody (ABIN125756) Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell le...
Product cited in: Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Background publications Schneider, Smith, Liu, Bismuth, Rudd: "CTLA-4 disrupts ZAP70 microcluster formation with reduced T cell/APC dwell times and calcium mobilization." in: European journal of immunology, Vol. 38, Issue 1, pp. 40-7, 2007 (PubMed).

Ishaq, DeGray, Natarajan: "Evidence for the involvement of tyrosine kinase ZAP 70 in nuclear retinoid receptor-dependent transactivation in T lymphocytes." in: The Journal of biological chemistry, Vol. 280, Issue 40, pp. 34152-8, 2005 (PubMed).

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