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CHEK2 antibody (pThr68)

CHEK2 Reactivity: Human, Monkey WB, ELISA, IF, IP, FACS, ICC Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN219984

Datasheet as PDF

Target See all CHEK2 Antibodies

CHEK2 (Checkpoint Kinase 2 (CHEK2))
Binding Specificity
26

23

19

16

16

15

15

15

9

8

8

8

6

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr68
Reactivity
234

87

73

16

12

7

6

5

2

2

2

1
Human, Monkey
Host
212

23

1
Rabbit
Compatible Secondaries
Clonality
206

30
Polyclonal
Conjugate
107

11

9

9

7

7

7

7

7

7

7

7

7

7

7

3

3

3

3

3

3

2

2

1
This CHEK2 antibody is un-conjugated
Application
198

79

78

67

39

34

30

28

23

18

16

5

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity

Recognizes endogenous levels of human Chk2 only when phosphorylated at threonine 68 (62kD). Does not cross-react with Chk2 phosphorylated at other sites. Species cross-reactivity: Monkey.

Purification

Protein A purified

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2 (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype

IgG

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anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody
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CHEK2 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

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CHEK2 Reactivity: Human, Dog WB, IHC, IF, IHC (p), IP, FACS Host: Mouse Monoclonal 5C4 unconjugated

Application Notes

Approved: ELISA, Flo (1:25), ICC (1:100), IF, IP (1:200), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Flow Cytometry. Western Blot: 1:1000. Immunocytochemistry: 1:100. Immunoprecipitation: 1:200. Flow Cytometry: 1:25.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative

Without preservative

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name

CHEK2 / CHK2 (CHEK2 Products)

Background

Name/Gene ID: CHEK2
Subfamily: CDS1
Family: Protein Kinase

Synonyms: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425

Gene ID

11200

UniProt

O96017

Pathways

p53 Signaling, Apoptosis, Cell Division Cycle