CD63 antibody (PE)

Catalog No. ABIN229869

Product Details anti-CD63 Antibody (PE)

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: Recognizes the CD63 Molecule of about 50kD.

Purification: Protein A purified

Immunogen: Human eosinophils.

Clone: AHN16-1-46-4-5

Isotype: IgG1

Application Details

Application Notes: Approved: Flo

Usage: Suitable for use in Flow Cytometry. FC: Flow Cytometry: 1:50 (or use 1.6 μL concentrated stock per 5x10e5 cells). 5x10e5 cultured HPB-MLT cells were washed and incubated 45 mins. on ice with 80 μL of LS-C35719 at a 1:50 dilution factor(20 μg/mL). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.70 log10fluorescent units when compared to a mouse IgG1/PE negativecontrol at a similar concentration. Binding was blocked when cellswere pre-incubated 10 minutes with 20 μL of 0.5 mg/mL anti-CD63antibody (Cat. #LS-C35716). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 15 % glycerol, 0.2 % BSA, 0.04 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Do not freeze. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing PE conjugates will result in a substantial loss of enzymatic activity.

Target Details for CD63

Target: CD63

Alternative Name: CD63 (CD63 Products)

Background: Name/Gene ID: CD63
Family: Tetraspan

Synonyms: CD63, CD63 Molecule, LAMP-3, ME491, Granulophysin, MLA1, Tetraspanin-30, Tspan-30, CD63 antigen, OMA81H, TSPAN30

Gene ID: 967

UniProt: P08962