CD63 antibody (APC)

Catalog No. ABIN238463

Product Details anti-CD63 Antibody (APC)

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The antibody MEM-259 reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Size Exclusion Chromatography

Immunogen: HPB-ALL T cell line

Clone: MEM-259

Isotype: IgG1

Application Details

Application Notes: Suitable for Flow Cytometry analysis of human blood cells using 10 m l reagent / 100 m l of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate Buffered Saline (PBS), 15 mM sodium azide, 0.2 % (w/v) high-grade BSA (Protease free)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD63

Target: CD63

Alternative Name: CD63 (CD63 Products)

Background: CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.Synonyms: Granulophysin, ME491, MLA1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, TSPAN30, Tetraspanin-30

Gene ID: 967

UniProt: P08962