Arylsulfatase A antibody (Internal Region)

Catalog No. ABIN238536

This anti-Arylsulfatase A antibody is a Goat Polyclonal antibody detecting Arylsulfatase A in WB, IHC, ELISA, FACS and IF. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 4+ publications.

Quick Overview for Arylsulfatase A antibody (Internal Region) (ABIN238536)

Target: Arylsulfatase A (ARSA)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Arylsulfatase A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Arylsulfatase A Antibody

Binding Specificity: Internal Region

Purpose: Arylsulfatase A

Sequence: YDLSKDPGEN YN

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YDLSKDPGENYN, from the internal region of the protein sequence according to NP_000478.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 60-65Da band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2,). This molecular weight is routinely observed by other sources and was successfully blocked by inc

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Arylsulfatase A antibody (ABIN238536)

Kanbe, Kamijo, Nakajima, Tanaka, Sugiyama, Wang, Fang, Hara, Gonzalez, Aoyama: "Chronic ethanol consumption decreases serum sulfatide levels by suppressing hepatic cerebroside sulfotransferase expression in mice." in: Archives of toxicology, Vol. 88, Issue 2, pp. 367-79, (2014) (PubMed).

Kimura, Nakajima, Kamijo, Tanaka, Wang, Hara, Sugiyama, Tanaka, Gonzalez, Aoyama: "Hepatic Cerebroside Sulfotransferase Is Induced by PPAR? Activation in Mice." in: PPAR research, Vol. 2012, pp. 174932, (2012) (PubMed).

Kiebish, Young, Lehman, Han: "Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1?-/- mouse cortex: a potential lipidomic role of PGC-1? in neurodegeneration." in: Journal of lipid research, Vol. 53, Issue 2, pp. 273-81, (2012) (PubMed).

Zhang, Nakajima, Kamijo, Li, Hu, Kannagi, Kyogashima, Aoyama, Hara: "Acute kidney injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside sulfotransferase in mice, resulting in reduction of liver and serum sulfatides." in: Biochemical and biophysical research communications, Vol. 390, Issue 4, pp. 1382-8, (2009) (PubMed).

Target Details for Arylsulfatase A

Target: Arylsulfatase A (ARSA)

Alternative Name: ARSA

Background: ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfatase

Gene ID: 410, 11883, 315222

NCBI Accession: NP_000478