CD8 alpha antibody (APC-Cy7)

Catalog No. ABIN2568039

The Mouse Monoclonal anti-CD8 alpha antibody has been validated for FACS. It is suitable to detect CD8 alpha in samples from Human.

Quick Overview for CD8 alpha antibody (APC-Cy7) (ABIN2568039)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to APC-Cy7

Application: Flow Cytometry (FACS)

Clone: RPA-T8

Product Details anti-CD8 alpha Antibody (APC-Cy7)

Specificity: The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 α/β) or as a homodimer (CD8 α/α) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells.

Purification: The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered aqueous solution, ≤0.09 % Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Storage: 4 °C

Storage Comment: The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze.

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: The RPA-T8 monoclonal antibody specifically reacts with the human CD8a molecule, a 32 kDa cell surface receptor expressed either as a heterodimer (CD8 α/β) or as a homodimer (CD8 α/α) on the majority of thymocytes, a subpopulation of mature T cells, and natural killer cells. CD8 interacts with the major histocompatibility complex class I (MHC class I) molecules on antigen-presenting cells or epithelial cells. The RPA-T8 antibody reacts with 13-48 % of peripheral lymphocytes, 80 % of thymocytes, and a subset of natural killer cells. RPA-T8, OKT8, and HIT8a antibodies do not compete with each other for binding to peripheral leukocytes, meaning that that they do not recognize the same epitope or block each other by steric hindrance.

Gene ID: 925

UniProt: P01732

Pathways: TCR Signaling