FOXP2 antibody (C-Term)

Catalog No. ABIN263146

This Goat Polyclonal antibody specifically detects FOXP2 in WB and ELISA. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for FOXP2 antibody (C-Term) (ABIN263146)

Target: FOXP2 (Forkhead Box P2 (FOXP2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-FOXP2 Antibody

Binding Specificity: C-Term

Purpose: FOXP2 (C terminus)

Sequence: REIEEEPLSE DLE

Specificity: This antibody is expected to recognise all three reported isoforms (NP_055306.1, NP_683696.2, NP_683697.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-REIEEEPLSEDLE, from the C Terminus of the protein sequence according to NP_055306.1, NP_683696.2, NP_683697.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80-90 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 82.6 kDa according to NP_683696.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2 μg/mL. Primary incubation

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FOXP2 antibody (ABIN263146)

Willett, Bayin, Lee, Krishnamurthy, Wojcinski, Lao, Stephen, Rosello-Diez, Dauber-Decker, Orvis, Wu, Tessier-Lavigne, Joyner: "Cerebellar nuclei excitatory neurons regulate developmental scaling of presynaptic Purkinje cell number and organ growth." in: eLife, Vol. 8, (2020) (PubMed).

Plaas, Seppa, Reimets, Jagomäe, Toots, Koppel, Vallisoo, Nigul, Heinla, Meier, Kaasik, Piirsoo, Hickey, Terasmaa, Vasar: "Wfs1- deficient rats develop primary symptoms of Wolfram syndrome: insulin-dependent diabetes, optic nerve atrophy and medullary degeneration." in: Scientific reports, Vol. 7, Issue 1, pp. 10220, (2019) (PubMed).

Haldipur, Dang, Aldinger, Janson, Guimiot, Adle-Biasette, Dobyns, Siebert, Russo, Millen: "Phenotypic outcomes in Mouse and Human Foxc1 dependent Dandy-Walker cerebellar malformation suggest shared mechanisms." in: eLife, Vol. 6, (2017) (PubMed).

Fujita, Morita, Furuichi, Sugihara: "Clustered fine compartmentalization of the mouse embryonic cerebellar cortex and its rearrangement into the postnatal striped configuration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 45, pp. 15688-703, (2012) (PubMed).

Fujita, Sugihara: "FoxP2 expression in the cerebellum and inferior olive: development of the transverse stripe-shaped expression pattern in the mouse cerebellar cortex." in: The Journal of comparative neurology, Vol. 520, Issue 3, pp. 656-77, (2011) (PubMed).

Target Details for FOXP2

Target: FOXP2 (Forkhead Box P2 (FOXP2))

Alternative Name: FOXP2

Background: FOXP2, forkhead box P2, SPCH1, CAGH44, TNRC10, CAG repeat protein 44, speech and language disorder 1, trinucleotide repeat containing 10, forkhead/winged-helix transcription factor, DKFZp686H1726, OTTHUMP00000196932

Gene ID: 93986

NCBI Accession: NP_055306, NP_683696, NP_683697