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CD25 antibody (Alexa Fluor 647)

Name: CD25 antibody (Alexa Fluor 647)
SKU: ABIN2657160
Availability: OutOfStock

IL2RA Reactivity: Human FACS Host: Mouse Monoclonal M-A251 Alexa Fluor 647

FACS

1 image

Catalog No. ABIN2657160

Datasheet as PDF

Target See all CD25 (IL2RA) Antibodies

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
Reactivity
168

117

36

10

10

5

3

2

2

2

1

1
Human
Host
138

83

80

2

2

1

1
Mouse
Compatible Secondaries
Clonality
231

70

4
Monoclonal
Conjugate
121

33

28

24

21

11

6

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD25 antibody is conjugated to Alexa Fluor 647
Application
225

86

56

53

44

40

23

18

15

13

8

8

6

6

4

2

2

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS)

Cross-Reactivity

Baboon, Cynomolgus, Rhesus Monkey

Purification

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.

Clone

M-A251

Isotype

IgG1 kappa

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Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Protect from prolonged exposure to light. Do not freeze.

Storage

4 °C

Storage Comment

The antibody solution should be stored undiluted between 2°C and 8°C
Target

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name

CD25 (IL2RA Products)

Background

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Pathways

JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation