IL-15 Protein (AA 49-162)
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- Target See all IL-15 (IL15) Proteins
- IL-15 (IL15) (Interleukin 15 (IL15))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 49-162
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- Western Blotting (WB), Immunofluorescence (IF)
- Purity
- Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin Level
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Less than 1 EU/μg cytokine as determined by the LAL method.
- Top Product
- Discover our top product IL15 Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Biological activity: ED50=2.5 - 15 ng/ml, corresponding to a specific activity of 0.6 - 4 x 105 units/mg, as determined by the dose dependent stimulation of CTLL-2 cell proliferation.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- Buffer
- Lyophilized from a concentrated solution (1 mg/mL) with 10 mM NaH2PO4, 150 mM NaCL, pH 8.0.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- Target
- IL-15 (IL15) (Interleukin 15 (IL15))
- Alternative Name
- IL-15 (IL15 Products)
- Synonyms
- IL15 Protein, AI503618 Protein, IL-15 Protein, interleukin 15 Protein, IL15 Protein, Il15 Protein
- Background
- IL-15 was discovered in the supernatant of Simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2. IL-15 is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, fibroblasts, and monocytes. IL-15 plays an important role in immune response and shares many functions with IL-2. For example, it stimulates the proliferation of activated T cells, NK cells, and B cells, and induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells and induces the production of proinflammatory cytokines from macrophages. IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares the receptor chains β and γc with IL-2. IL-15 is normally not secreted in soluble form but is instead held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 is then presented in trans form to T cells and NK cells and is recognized by the γc receptor on these cells, such recognition maintains cell survival and intermittent proliferation.
- Molecular Weight
- The 115 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 13,382 Da.
- Pathways
- JAK-STAT Signaling, Glycosaminoglycan Metabolic Process
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