TNFSF9 antibody

Catalog No. ABIN2688839

This Rat Monoclonal antibody specifically detects TNFSF9 in FACS and BR. It exhibits reactivity toward Mouse. It has been mentioned in 4+ publications

Quick Overview for TNFSF9 antibody (ABIN2688839)

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFSF9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: TKS

Product Details anti-TNFSF9 Antibody

Characteristics: The TKS-1 antibody reacts with 4-1BB Ligand (4-1BBL), a 97 kDa member of the NGF/TNF superfamily which is present on antigen-presenting cells and activated B lymphocytes. 4-1BBL interacts with 4-1BB (CDw137), found predominantly on activated T lymphocytes. This ligand-receptor pair is grouped with pairs such as CD27/CD70, CD30/CD153, OX-40/OX-40L, CD80/CD28, and CD86/CD28, which costimulate T-cell proliferation and cytokine production. The interaction of 4-1BB with 4-1BBL may be reciprocally costimulatory in that 4-1BBL may deliver proliferative signals to B lymphocytes activated by anti-IgM. The TKS-1 mAb stains B cells activated for three days with anti-IgM polyclonal antibody, consistent with an earlier report that soluble 4-1BB-alkaline phosphatase fusion protein binds to anti-IgM-activated B cells. Furthermore, 4-1BBL mRNA has been detected in activated T lymphocytes and activated macrophages. The TKS-1 mAb has been reported to inhibit the binding of soluble 4-1BB to 4-1BBL transfectants and block the in vitro costimulation of T-lymphocyte proliferation by 4-1BBL transfectants. Expression of 4-1BB Ligand (4-1BBL) on mouse splenocytes. C57BL/6 splenocytes, freshly isolated (left two panels) and following 3-day activation with soluble anti-mouse IgM (Jackson Immunoresearch, right two panels), were stained with purified rat IgG2a, κ isotype control mAb R35-95 (Cat. no. 553927, far left and center right panels) or purified mAb TKS-1 (center left and far right panels), followed by PE-conjugated goat anti-rat Ig (Cat. no. 550767, all panels), then FITC-conjugated anti-mouse CD45R/B220 mAb RA3-6B2 (Cat. no. 553087/553088, all panels). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse 4-1BB Ligand - Purified - Clone TKS-1 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse B lymphoma line 2PK-3

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TNFSF9 antibody (ABIN2688839)

Akiba, Miyahira, Atsuta, Takeda, Nohara, Futagawa, Matsuda, Aoki, Yagita, Okumura: "Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis." in: The Journal of experimental medicine, Vol. 191, Issue 2, pp. 375-80, (2000) (PubMed).

DeBenedette, Shahinian, Mak, Watts: "Costimulation of CD28- T lymphocytes by 4-1BB ligand." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 551-9, (1997) (PubMed).

Pollok, Kim, Kwon: "Regulation of 4-1BB expression by cell-cell interactions and the cytokines, interleukin-2 and interleukin-4." in: European journal of immunology, Vol. 25, Issue 2, pp. 488-94, (1995) (PubMed).

Goodwin, Din, Davis-Smith, Anderson, Gimpel, Sato, Maliszewski, Brannan, Copeland, Jenkins: "Molecular cloning of a ligand for the inducible T cell gene 4-1BB: a member of an emerging family of cytokines with homology to tumor necrosis factor." in: European journal of immunology, Vol. 23, Issue 10, pp. 2631-41, (1993) (PubMed).

Target Details for TNFSF9

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: 4-1BB Ligand

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints