FOXP3 antibody (AA 101-431)

Catalog No. ABIN3042405

This anti-FOXP3 antibody is a Rabbit Polyclonal antibody detecting FOXP3 in IHC. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 10+ publications.

Quick Overview for FOXP3 antibody (AA 101-431) (ABIN3042405)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-FOXP3 Antibody

Binding Specificity: AA 101-431

Purpose: Anti-Forkhead box protein P3 FOXP3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Forkhead box protein P3 FOXP3 Antibody (ABIN3042405). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FOXP3 recombinant protein (Position: H101-P431). Human FOXP3 shares 88% amino acid (aa) sequence identity with mouse FOXP3.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
1. Allan, S. E., Passerini, L., Bacchetta, R., Crellin, N., Dai, M., Orban, P. C., Ziegler, S. F., Roncarolo, M. G., Levings, M. K.The role of 2 FOXP3 isoforms in the generation of human CD4+ Tregs.J. Clin. Invest. 115: 3276-3284, 2005. 2. Bennett, C. L., Christie, J., Ramsdell, F., Brunkow, M. E., Ferguson, P. J., Whitesell, L., Kelly, T. E., Saulsbury, F. T., Chance, P. F., Ochs, H. D.The immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) is caused by mutations of FOXP3.Nature Genet. 27: 20-21, 2001. 3. Hong, J., Li, N., Zhang, X., Zheng, B., Zhang, J. Z.Induction of CD4+CD25+ regulatory T cells by copolymer-I through activation of transcription factor Foxp3.Proc. Nat. Acad. Sci. 102: 6449-6454, 2005.

Comment:

Antibody can be supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-FOXP3 antibody (ABIN3042405)

Li, Chen, Xie: "Decreased FOXP3+ and GARP+ Tregs to neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer." in: OncoTargets and therapy, Vol. 9, pp. 3525-33, (2016) (PubMed).

Tao, Zhu, Chen, Wang, Pan, Yu, Zhou, Zhu: "IL-35 improves Treg-mediated immune suppression in atherosclerotic mice." in: Experimental and therapeutic medicine, Vol. 12, Issue 4, pp. 2469-2476, (2016) (PubMed).

Gong, Wang, Yuan, Li, Gu, Zhao, Zhang, Jia, Feng, Liu: "Inhibition of Tumor Growth and Immunomodulatory Effects of Flavonoids and Scutebarbatines of Scutellaria barbata D. Don in Lewis-Bearing C57BL/6 Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 630760, (2015) (PubMed).

Li, Ren, Wang, Gu, Hu, Ren, Hong, Wu, Liu, Li: "T2 enhances in situ level of Foxp3+ regulatory cells and modulates inflammatory cytokines in Crohn's disease." in: International immunopharmacology, Vol. 18, Issue 2, pp. 244-8, (2014) (PubMed).

Wang, Dai, Dong, Sun, Song, Guo, Zhu, Wang, Zhang: "The modulation of endoplasmic reticulum stress by chemical chaperone upregulates immune negative cytokine IL-35 in apolipoprotein E-deficient mice." in: PLoS ONE, Vol. 9, Issue 1, pp. e87787, (2014) (PubMed).

Gai, Li, Song, Lei, Yang: "In situ analysis of FOXP3(+) regulatory T cells and myeloid dendritic cells in human colorectal cancer tissue and tumor-draining lymph node." in: Biomedical reports, Vol. 1, Issue 2, pp. 207-212, (2014) (PubMed).

Hu, Li, Maskey, Xu, Peng, Wang, Li, Yang: "Decreased intratumoral Foxp3 Tregs and increased dendritic cell density by neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4685-94, (2014) (PubMed).

Qiao, Huang, Xu, Zhao, Ran, Shen: "LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn's disease." in: Journal of biomedical science, Vol. 20, pp. 87, (2014) (PubMed).

Fu, Li, Yang, Gai, Jia, Lei, Li: "FOXP3 and TLR4 protein expression are correlated in non-small cell lung cancer: implications for tumor progression and escape." in: Acta histochemica, Vol. 115, Issue 2, pp. 151-7, (2013) (PubMed).

Gai, Song, Li, Lei, Yang: "Potential role of plasmacytoid dendritic cells for FOXP3+ regulatory T cell development in human colorectal cancer and tumor draining lymph node." in: Pathology, research and practice, Vol. 209, Issue 12, pp. 774-8, (2013) (PubMed).

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3

Background:

Synonyms: Forkhead box protein P3,Scurfin,Forkhead box protein P3, C-terminally processed,Forkhead box protein P3 41 kDa form,FOXP3,IPEX,JM2,

Background: FOXP3 (forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome (specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.

Sequence Similarities: Contains 1 C2H2-type zinc finger.

Molecular Weight: 78 kDa

Gene ID: 50943

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation