EIF4E3 antibody (Middle Region)

Catalog No. ABIN310121

The Rabbit Polyclonal anti-EIF4E3 antibody has been validated for WB. It is suitable to detect EIF4E3 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). There is 1 publication available.

Quick Overview for EIF4E3 antibody (Middle Region) (ABIN310121)

Target: EIF4E3 (Eukaryotic Translation Initiation Factor 4E Family Member 3 (EIF4E3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF4E3 Antibody

Binding Specificity: Middle Region

Sequence: VQVWNVNASL VGEATVLEKI YELLPHITFK AVFYKPHEEH HAFEGGRGKH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4E3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF4E3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 224 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF4E3 antibody (ABIN310121)

Yi, Papadopoulos, Hagner, Wagner: "Hypoxia-inducible factor-1? (HIF-1?) promotes cap-dependent translation of selective mRNAs through up-regulating initiation factor eIF4E1 in breast cancer cells under hypoxia conditions." in: The Journal of biological chemistry, Vol. 288, Issue 26, pp. 18732-42, (2013) (PubMed).

Target Details for EIF4E3

Target: EIF4E3 (Eukaryotic Translation Initiation Factor 4E Family Member 3 (EIF4E3))

Alternative Name: EIF4E3

Background: EIF4E3 belongs to the EIF4E family of translational initiation factors that interact with the 5-prime cap structure of mRNA and recruit mRNA to the ribosome.
Alias Symbols: MGC39820, MGC86971
Protein Interaction Partner: ELAVL1, DCC,
Protein Size: 224

Molecular Weight: 24 kDa

Gene ID: 317649

NCBI Accession: NM_001134649, NP_001128123

UniProt: Q8N5X7