EIF4E3 antibody (Middle Region)

Catalog No. ABIN310121

Product Details anti-EIF4E3 Antibody

Target: EIF4E3 (Eukaryotic Translation Initiation Factor 4E Family Member 3 (EIF4E3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VQVWNVNASL VGEATVLEKI YELLPHITFK AVFYKPHEEH HAFEGGRGKH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF4E3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF4E3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 224 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF4E3 antibody (ABIN310121)

Yi, Papadopoulos, Hagner, Wagner: "Hypoxia-inducible factor-1? (HIF-1?) promotes cap-dependent translation of selective mRNAs through up-regulating initiation factor eIF4E1 in breast cancer cells under hypoxia conditions." in: The Journal of biological chemistry, Vol. 288, Issue 26, pp. 18732-42, (2013) (PubMed).

Target Details for EIF4E3

Target: EIF4E3 (Eukaryotic Translation Initiation Factor 4E Family Member 3 (EIF4E3))

Alternative Name: EIF4E3 (EIF4E3 Products)

Background: EIF4E3 belongs to the EIF4E family of translational initiation factors that interact with the 5-prime cap structure of mRNA and recruit mRNA to the ribosome.
Alias Symbols: MGC39820, MGC86971
Protein Interaction Partner: ELAVL1, DCC,
Protein Size: 224

Molecular Weight: 24 kDa

Gene ID: 317649

NCBI Accession: NM_001134649, NP_001128123

UniProt: Q8N5X7