COPB1 antibody (Middle Region)

Catalog No. ABIN311506

This Rabbit Polyclonal antibody specifically detects COPB1 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit and Zebrafish (Danio rerio)and has been mentioned in 1 publication.

Quick Overview for COPB1 antibody (Middle Region) (ABIN311506)

Target: COPB1 (Coatomer Protein Complex, Subunit beta 1 (COPB1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COPB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-COPB1 Antibody

Binding Specificity: Middle Region

Sequence: QLALDLVSSR NVEELVIVLK KEVIKTNNVS EHEDTDKYRQ LLVRTLHSCS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against COPB1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human COPB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 953 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-COPB1 antibody (ABIN311506)

Goryachev, Pokhilko: "Computational model explains high activity and rapid cycling of Rho GTPases within protein complexes." in: PLoS computational biology, Vol. 2, Issue 12, pp. e172, (2007) (PubMed).

Target Details for COPB1

Target: COPB1 (Coatomer Protein Complex, Subunit beta 1 (COPB1))

Alternative Name: COPB1

Background: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins, the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Alias Symbols: COPB, DKFZp761K102, FLJ10341
Protein Interaction Partner: SUMO2, NUDCD1, UBC, RNF2, EZH2, KLC1, DIAPH1, ARCN1, ACACA, COPG1, COPZ1, TLE3, FBXO6, UBD, KCNK3, KCNK9, NPM1, HDAC10, ERBB2IP, SNTA1, LZTS2, VPS18, NUB1, SERTAD1, FOXB1, IRAK3, TANK, ID1, HLA-DPB1, GRB2, XRCC6, FN1, CFTR, FMNL1, COPG2, COPE, COPB2, COPA
Protein Size: 953

Molecular Weight: 107 kDa

Gene ID: 1315

NCBI Accession: NM_016451, NP_057535

UniProt: P53618