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CD8 antibody (FITC)

CD8 Reactivity: Mouse FACS Host: Rat Monoclonal 07-06-53 FITC

Catalog No. ABIN317090

Datasheet as PDF

Target See all CD8 Antibodies

CD8
Reactivity
197

66

25

12

12

12

9

9

8

8

6

6

5

5

5

4

4

4

3

1
Mouse
Host
229

48

36

4
Rat
Compatible Secondaries
Clonality
280

30

2
Monoclonal
Conjugate
102

48

37

25

12

6

5

5

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 antibody is conjugated to FITC
Application
279

67

55

51

50

44

30

21

18

17

15

14

8

5

5

4

3

2

1

1

1

1
Flow Cytometry (FACS)

Specificity

This antibody detects CD8a, the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. This antibody Clone 53-6.7 has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells.

Characteristics

Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen

Mouse thymus or spleen.

Clone

07-06-53

Isotype

IgG2a

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anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PE

anti-CD8 (CD8) (AA 22-187) antibody
CD8 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD8 (CD8) antibody
CD8 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-31 unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Cat FACS Host: Mouse Monoclonal fCD8 PE

anti-CD8 (CD8) antibody
CD8 Reactivity: Human IHC, IP, WB, ELISA Host: Rabbit Monoclonal 3C3 unconjugated Recombinant Antibody

anti-CD8 (CD8) antibody (Biotin)
CD8 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-31 Biotin

anti-CD8 (CD8) antibody (FITC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 FITC

anti-CD8 (CD8) antibody (PerCP)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PerCP

anti-CD8 (CD8) antibody (APC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 APC

Application Notes

Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.5mg/mL

Buffer

PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.

Expiry Date

12 months
Target

CD8

Alternative Name

CD8 (CD8 Products)

Background

CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID

12525

UniProt

P01731