RHCE antibody

Catalog No. ABIN319446

Product Details anti-RHCE Antibody

Target: RHCE (Rhesus Blood Group, CcEe Antigens (RHCE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RHCE antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: This antibody recognises erythrocytes of normal Rh types but does not recognise Rhnull erythrocytes. It reacts with 31 kDa and 35-51 kDa proteins which were designated CD240DCE at the 7th leucocyte typing workshop.

Cross-Reactivity (Details): Also reacts to: CD240D

Purification: Purified

Immunogen: Human erythrocytes

Clone: BRIC69

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation. Flow Cytometry: 10l of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Tris buffered glycine, pH 7.7 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RHCE

Target: RHCE (Rhesus Blood Group, CcEe Antigens (RHCE))

Alternative Name: CD240CE / RHCE (RHCE Products)

Background: The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene which encodes both the RhC and RhE antigens on a single polypeptide and a second gene which encodes the RhD protein. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. A mutation in this gene results in amorph-type Rh-null disease. There are thirteen named isoforms.Synonyms: Blood group Rh(CE) polypeptide, Rh polypeptide 1, Rh30A, RhIXB, Rhesus C/E antigens

Gene ID: 6006

NCBI Accession: NP_065231

UniProt: P18577