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CD8 antibody

CD8 Reactivity: Mouse FACS, IHC (fro) Host: Rat Monoclonal YTS105-18 unconjugated

Catalog No. ABIN319569

Datasheet as PDF

Target See all CD8 Antibodies

CD8
Reactivity
196

63

25

12

12

12

9

9

8

7

6

6

5

5

5

4

4

4

3

1
Mouse
Host
226

47

36

4
Rat
Compatible Secondaries
Clonality
276

30

2
Monoclonal
Conjugate
100

48

37

26

13

6

6

5

5

5

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8 antibody is un-conjugated
Application
275

66

55

50

49

47

30

21

18

17

15

14

8

5

5

4

3

2

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

SM024LE recognises a non polymorphic epitope on the mouse CD8 alpha chain. Antibodies produced by clone YTS105.18 have been reported to block MHC I dependent T-cell responses in vitro and in vivo, and induce transplantation tolerance in combination with CD4 antibodies.

Purification

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Endotoxin Level

Low Endotoxin

Immunogen

Mouse spleen cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.

Clone

YTS105-18

Isotype

IgG2a

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CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PE

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CD8 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

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CD8 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-31 unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Cat FACS Host: Mouse Monoclonal fCD8 PE

anti-CD8 (CD8) antibody
CD8 Reactivity: Human IHC, WB, IP, ELISA Host: Rabbit Monoclonal 3C3 unconjugated Recombinant Antibody

anti-CD8 (CD8) antibody (Biotin)
CD8 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-31 Biotin

anti-CD8 (CD8) antibody (FITC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 FITC

anti-CD8 (CD8) antibody (PerCP)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PerCP

anti-CD8 (CD8) antibody (APC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 APC

Application Notes

Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.4 without preservatives.

Preservative

Without preservative

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Target

CD8

Alternative Name

CD8 (CD8 Products)

Background

CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds. It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID

12525

NCBI Accession

NP_001074579

UniProt

P01731