This antibody recognizes the Human CD32 antigen. Clone AT10 blocks the binding of IgG to Fc gamma R11 (See Larsson, M. et al. for details). SM1092LE is recommended for this purpose.
Purification
Affinity chromatography on Protein A
Immunogen
K562 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
FCGR2A
Reactivity: Human
WB
Host: Mouse
Polyclonal
unconjugated
Application Notes
Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL distilled water.
Buffer
PBS, 0.09 % Sodium Azide, 1 % BSA, 5 % sucrose
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Target
FCGR2A
(Fc Fragment of IgG, Low Affinity IIa, Receptor (CD32) (FCGR2A))
Alternative Name
CD32 / FcRII-A
Background
CD32 is a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation.CD32 is expressed by B cells, monocytes, granulocytes and platelets.Synonyms: CDw32, FCG2, FCGR2A, FCGR2A1, Fc-gamma RII-a, Fc-gamma-RIIa, IGFR2, IgG Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II