Integrin beta 4 antibody

Catalog No. ABIN319862

Product Details anti-Integrin beta 4 Antibody

Target: Integrin beta 4 (ITGB4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with beta 4 Integrin (CD104). This antibody recognises an extracellular epitope on the CD104 Molecule.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified alpha 6/beta 4 Integrin from A431 cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Clone: 450-9D

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (1/25-1/100): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Western blot. Immunohistochemistry on Frozen Sections (1/500-1/1000). Does not work on Paraffin Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: CD104 / Integrin beta-4 (ITGB4 Products)

Background: Integrin beta 4 is a glycoprotein which associates with the a6 integrin to form the a6/b4 complex. Integrin alpha 6/beta 4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. Defects in Integrin beta 4 gene are a cause of epidermolysis bullosa letalis with pyloric atresia (EB PA), also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is characterized by mucocutaneous fragility and gastrointestinal atresia, which most commonly affects the pylorus. Moreover, defects in Integrin beta 4 gene are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). This nonlethal form of junctional epidermolysis bullosa is characterized by life long blistering of the skin, associated with hair and tooth abnormalities. CD104 is expressed on epithelial cells, Schwann cells and some tumor cell lines.Synonyms: GP150, ITGB4

Gene ID: 3691

NCBI Accession: NP_000204

UniProt: P16144

Pathways: Integrin Complex