CD90 antibody
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- Target See all CD90 (THY1) Antibodies
- CD90 (THY1)
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD90 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
- Specificity
- This antibody recognises the Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. This clone recognises a 30 kDa glycoprotein in Immunoprecipitation and Western blotting assays. The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.
- Purification
- Affinity Chromatography on Protein G.
- Immunogen
- EL-4 cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line.
- Clone
- FF-10
- Isotype
- IgG1
- Top Product
- Discover our top product THY1 Primary Antibody
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anti-CD90 (THY1) (AA 31-120) antibody
THY1 Reactivity: Human, Mouse, Rat, Cow, Dog, Sheep FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated
anti-CD90 (THY1) (AA 17-132) antibodyTHY1 Reactivity: Human WB, IHC, ELISA, ICC Host: Mouse Monoclonal 7E1B11 unconjugated
anti-CD90 (THY1) (AA 20-133) antibodyTHY1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD90 (THY1) (AA 36-65), (N-Term) antibodyTHY1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB03970 unconjugated
anti-CD90 (THY1) antibodyTHY1 Reactivity: Human, Monkey, Baboon FACS, IHC, IHC (fro), IP, IHC (p) Host: Mouse Monoclonal 5E10 unconjugated
anti-CD90 (THY1) (AA 1-161) antibodyTHY1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3F9 unconjugated
anti-CD90 (THY1) (AA 20-131) antibodyTHY1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-CD90 (THY1) antibody (FITC)THY1 Reactivity: Human, Pig, Horse, Non-Human Primate FACS Host: Mouse Monoclonal 5E10 FITC
anti-CD90 (THY1) (Internal Region) antibodyTHY1 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-CD90 (THY1) antibodyTHY1 Reactivity: Mouse FACS, CyTox Host: Mouse Monoclonal F7D5 unconjugated
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- Application Notes
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Flow Cytometry (1/50-1/100). Immunohistochemistry on Frozen Sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD90 (THY1)
- Alternative Name
- CD90 (THY1 Products)
- Background
- CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
- Gene ID
- 21838
- NCBI Accession
- NP_033408
- UniProt
- P01831
- Pathways
- Cell-Cell Junction Organization
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