CD48 antibody

Catalog No. ABIN323355

Product Details anti-CD48 Antibody

Target: CD48

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD48 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Specificity: Recognizes human CD48. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmCD48 or rhOX40.

Purification: Protein G purified

Immunogen: NS0-derived rhCD48.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, Flo, WB

Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human CD48. Using a colorimetric detection system, the detection limit for rhCD48 is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human CD48. The detection limit for rhCD48 is approximately 10 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for CD48

Target: CD48

Alternative Name: CD48 (CD48 Products)

Background: Name/Gene ID: CD48

Synonyms: CD48, BCM1, BCM1 surface antigen, BLAST, BLAST1, CD48 Molecule, MEM-102, MCD48, SLAMF2, CD48 antigen, HCD48, Leukocyte antigen MEM-102, TCT.1

Gene ID: 962

UniProt: P09326