TNFSF9 antibody (Extracellular Domain)

Catalog No. ABIN331664

Product Details anti-TNFSF9 Antibody

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Binding Specificity: Extracellular Domain

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFSF9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Specificity: Recognizes mouse 4-1BB Ligand. In direct ELISA, does not cross-react with rhRELT.

Purification: Protein G purified

Immunogen: Sf 21-derived rm4-1BB Ligand extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, Flo, WB

Usage: Western Blot, Flow Cytometry, Direct ELISA. Western Blot-This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect mouse 4-1 BB Ligand. The detection limit for rm4-1BB Ligand is approximately 1 ng/lane under non-reducing and reducing conditions. Flow Cytometry-Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse 4-1 BB Ligand. The detection limit for rm4-1BB Ligand is approximately 1.5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized powder at 4°C for short term only. Reconstitute with sterile 40-50% glycerol and store at -20°C.

Target Details for TNFSF9

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: TNFSF9 / CD137L (TNFSF9 Products)

Background: Name/Gene ID: TNFSF9
Family: TNF

Synonyms: TNFSF9, 4-1BBL, 4-1BB ligand, 4-1BB-L, CD137L, Homolog of mouse 4-1BB-L, Receptor 4-1BB ligand

Gene ID: 8744

UniProt: P41273

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints