Fascin 2 antibody (Internal Region)

Catalog No. ABIN334397

The Goat Polyclonal anti-Fascin 2 antibody has been validated for IHC and ELISA. It is suitable to detect Fascin 2 in samples from Mouse. There are 3+ publications available.

Quick Overview for Fascin 2 antibody (Internal Region) (ABIN334397)

Target: Fascin 2 (FSCN2)

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Fascin 2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Fascin 2 Antibody

Binding Specificity: Internal Region

Purpose: Fascin 2

Sequence: CHHRGSNQLD TNR

Specificity: This antibody is expected to recognize both reported isoforms (NP_001070650.1 and NP_036550.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CHHRGSNQLDTNR, from the internal region of the protein sequence according to NP_001070650.1, NP_036550.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Mouse Brain. Recommended concentration: 4 μg/mL. This product was successfully used on sections of mouse cochlea as descibed in Shin et al, J Neurosci. 2010 Jul 21,30(29):9683-94, PMID: 20660251.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Fascin 2 antibody (ABIN334397)

Burns, Kelly, Hoa, Morell, Kelley: "Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear." in: Nature communications, Vol. 6, pp. 8557, (2015) (PubMed).

Ramakrishnan, Drescher, Khan, Hatfield, Drescher: "HCN1 and HCN2 proteins are expressed in cochlear hair cells: HCN1 can form a ternary complex with protocadherin 15 CD3 and F-actin-binding filamin A or can interact with HCN2." in: The Journal of biological chemistry, Vol. 287, Issue 45, pp. 37628-46, (2012) (PubMed).

Shin, Longo-Guess, Gagnon, Saylor, Dumont, Spinelli, Pagana, Wilmarth, David, Gillespie, Johnson: "The R109H variant of fascin-2, a developmentally regulated actin crosslinker in hair-cell stereocilia, underlies early-onset hearing loss of DBA/2J mice." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 29, pp. 9683-94, (2010) (PubMed).

Target Details for Fascin 2

Target: Fascin 2 (FSCN2)

Alternative Name: FSCN2

Background: Fascin homolog 2, actin-bundling protein, retinal (Strongylocentrotus purpuratus), RFSN, RP30, fascin 2, retinal fascin,

Gene ID: 25794, 238021, 303741

NCBI Accession: NP_001070650, NP_036550