Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (Internal Region) antibody

Details for Product No. ABIN334459
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Antigen
Synonyms CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha, 6330412C24Rik, caPI3K, p110, p110alpha, C-P3K, PI3-kinase, PI3K-alpha, pi3-k, pi3k
Epitope
Internal Region
(12), (12), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(78), (42), (37), (14), (12), (2), (1)
Host
Goat
(66), (8), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(42), (35), (28), (20), (14), (6), (6), (6), (4), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN334459
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence according to NP_006209.2.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PIK3CA / P110alpha
Background Synonyms: PIK3CA, p110-alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide, MGC142161, MGC142163, PI3K, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
Gene ID 18706
NCBI Accession NP_006209.2
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration: 2-3 µg/mL. This antibody was successfully used on Human epithelial ovarian carcinoma: Abubaker et al. Mol Cancer. 2009 Jul 28,8:51. (PMID: 19638206).
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Abubaker, Bavi, Al-Haqawi et al.: "PIK3CA alterations in Middle Eastern ovarian cancers." in: Molecular cancer, Vol. 8, pp. 51, 2009 (PubMed).

General Lopez-Knowles, Hernandez, Malats et al.: "PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors." in: Cancer research, Vol. 66, Issue 15, pp. 7401-4, 2006 (PubMed).

Hosts (66), (8), (6)
Reactivities (78), (42), (37), (14), (12), (2), (1)
Applications (42), (35), (28), (20), (14), (6), (6), (6), (4), (1), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (12), (12), (2), (2), (2), (2), (1), (1), (1), (1)
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