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PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (Internal Region)

Details for Product anti-PIK3CA Antibody No. ABIN334459, Supplier: Log in to see
Antigen
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
Alternatives
anti-Human PIK3CA antibody for ELISA
Epitope
Internal Region
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16
14
14
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12
9
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
154
49
43
7
4
2
1
1
1
Host
Goat
113
23
21
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
7
7
7
6
5
5
2
2
2
2
2
2
2
2
2
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
90
73
66
32
22
11
8
5
5
3
2
2
1
1
Supplier
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Immunogen Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence according to NP_006209.2.
Blocking Peptide Blocking peptide for this product available: ABIN370308
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PIK3CA / P110alpha (PIK3CA Antibody Abstract)
Background Synonyms: PIK3CA, p110-alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide, MGC142161, MGC142163, PI3K, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
Gene ID 18706
NCBI Accession NP_006209
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration: 2-3 µg/mL. This antibody was successfully used on Human epithelial ovarian carcinoma: Abubaker et al. Mol Cancer. 2009 Jul 28,8:51. (PMID: 19638206).
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Product cited in: Abubaker, Bavi, Al-Haqawi et al.: "PIK3CA alterations in Middle Eastern ovarian cancers." in: Molecular cancer, Vol. 8, pp. 51, 2009 (PubMed).

Background publications Lopez-Knowles, Hernandez, Malats et al.: "PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors." in: Cancer research, Vol. 66, Issue 15, pp. 7401-4, 2006 (PubMed).