Actin, gamma 2, Smooth Muscle, Enteric (ACTG2) antibody

Antigen: Actin, gamma 2, Smooth Muscle, Enteric (ACTG2)

Synonyms: ACT, ACTE, ACTA3, ACTL3, ACTSG, Act4, SMGA, Act-4, ACTG2, MGC129006, GAMMA-ACTIN, ACTGE, MGC105507

Clonality: Monoclonal (Alpha-SM1-1A4)

Host: Mouse

Reactivity: Human, Rat (Rattus), Chicken, Goat, Rabbit, Mouse (Murine)

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoelectron Microscopy (EM), ELISA

Catalog no.: ABIN335372

Additional Information

Alternative name: alpha-smooth muscle actin

Immunogen: alpha-SM1 (clone 1A4) is a mouse monoclonal IgG2a antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a peptide comprising the first 10 amino acids of alpha-smooth muscle actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin via the C-terminal cysteine (Ac-EEEDSTALVC).

Isotype: IgG2a

Clone: Alpha-SM1-1A4

Description: Among the six actin isoforms described in mammals, two are found in virtually all cells (betaˆ’ and gammaˆ’cytoplasmic), two are detected in smooth muscle cells (alphaˆ’ and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus and the sequences of each of them are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown to appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases.

Specificity: The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals (amongst others human, rat, chicken, goat, rabbit, mouse).

Application Details

Application Notes: alpha-SM1 reacts exclusively with alpha-smooth muscle actin which is typical for vascular and visceral smooth muscle cells, but which is also present in myofibroblasts. The epitope that is recognized by alpha-SM1 is Ac-EEED. alpha-SM1 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting, immuno- electron microscopy and ELISA. Optimal antibody dilution should be determined by titration, recommended range is 1:1000 - 1:10.000 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:10.000 - 1:50.000 for immunoblotting applications.

Purification: Purified

Storage: Store at 4 o C, do not freeze.

Restrictions: For Research Use only

Images

anti-Actin, gamma 2, Smooth Muscle, Enteric (ACTG2) antibody

anti-Actin, gamma 2, Smooth Muscle, Enteric (ACTG2) antibody (Image 2)

anti-Actin, gamma 2, Smooth Muscle, Enteric (ACTG2) antibody (Image 3)

Publications

Skalli, Schuerch, Seemayer et al.: "Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 60, Issue 2, pp. 275-85, 1989 (PubMed).

Skalli, Ropraz, Trzeciak et al.: "A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 2, pp. 2787-96, 1987 (PubMed).

Vyalov, Gabbiani, Kapanci: "Rat alveolar myofibroblasts acquire alpha-smooth muscle actin expression during bleomycin-induced pulmonary fibrosis." in: The American journal of pathology, Vol. 143, Issue 6, pp. 1754-65, 1994 (PubMed).

Chaponnier, Goethals, Janmey et al.: "The specific NH2-terminal sequence Ac-EEED of alpha-smooth muscle actin plays a role in polymerization in vitro and in vivo." in: The Journal of cell biology, Vol. 130, Issue 4, pp. 887-95, 1995 (PubMed).

Simoncelli, Fagotti, Di Rosa et al.: "Expression of an actin in protochordates and lower craniates defined by anti-alpha SM-1." in: European journal of cell biology, Vol. 69, Issue 3, pp. 297-300, 1996 (PubMed).