Smooth Muscle Actin antibody (AA 1-377)

Catalog No. ABIN513087

Cited in 2+ publications. The Mouse Monoclonal anti-Smooth Muscle Actin antibody (Clone 4A8-2H3) (ABIN513087) specifically detects Smooth Muscle Actin in WB, IF and ELISA. The antibody is reactive with Human samples.

Quick Overview for Smooth Muscle Actin antibody (AA 1-377) (ABIN513087)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Clone: 4A8-2H3

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: AA 1-377

Purpose: Mouse monoclonal antibody raised against a full length recombinant ACTA2.

Sequence: MCEEEDSTAL VCDNGSGLCK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH SFYNELRVAP EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS LSTFQQMWIS KQEYDEAGPS IVHRKCF

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: ACTA2 (AAH17554.1, 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-Smooth Muscle Actin antibody (ABIN513087)

Lino Cardenas, Henaoui, Courcot, Roderburg, Cauffiez, Aubert, Copin, Wallaert, Glowacki, Dewaeles, Milosevic, Maurizio, Tedrow, Marcet, Lo-Guidice, Kaminski, Barbry, Luedde, Perrais, Mari, Pottier: "miR-199a-5p Is upregulated during fibrogenic response to tissue injury and mediates TGFbeta-induced lung fibroblast activation by targeting caveolin-1." in: PLoS genetics, Vol. 9, Issue 2, pp. e1003291, (2013) (PubMed).

Van der Hauwaert, Savary, Gnemmi, Glowacki, Pottier, Bouillez, Maboudou, Zini, Leroy, Cauffiez, Perrais, Aubert: "Isolation and characterization of a primary proximal tubular epithelial cell model from human kidney by CD10/CD13 double labeling." in: PLoS ONE, Vol. 8, Issue 6, pp. e66750, (2013) (PubMed).

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: ACTA2

Background: Full Gene Name: actin, alpha 2, smooth muscle, aorta
Synonyms: AAT6,ACTSA

Gene ID: 59

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development