Smooth Muscle Actin antibody (N-Term)

Catalog No. ABIN6940521

The Mouse Monoclonal anti-Smooth Muscle Actin antibody is suitable to detect Smooth Muscle Actin in samples from Human and Rat. It has been validated for IHC, IF, FACS and StM.

Quick Overview for Smooth Muscle Actin antibody (N-Term) (ABIN6940521)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: 1A4-ACTA2-791

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: N-Term

Specificity: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Cross-Reactivity (Details): Predicted to show a broad reactivity.

Purification: Purified by Protein A/G

Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH (1A4), Recombinant full-length human ACTA2 protein (ACTA2/791)

Isotype: IgG2a kappa

Application Details

Application Notes:

Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: ACTA2

Molecular Weight: 42kDa

Gene ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development