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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Potassium Inwardly-Rectifying Channel, Subfamily J, Member 12 (KCNJ12) (Cytoplasmic Domain) antibody
|Synonyms||IRK2, hIRK, hIRK1, KCNJN1, Kir2.2, Kir2.2v, kcnj12x, FLJ14167, hkir2.2x, MB-IRK2, MGC144838, MGC144839, Kir2.1, MGC156606, IRK|
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|5 references available|
|Quantity||500 µg (Variants)|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||KCNJ12 (KCNJN1, Kir2.2, IRK2)|
|Immunogen||A synthetic peptide from the cytoplasmic domain of human KCNJ12 (KCNJN1, Kir2.2, IRK2) conjugated to an immunogenic carrier protein was used as the immunogen.|
|Description||FUNCTION: Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Tissue specificity: Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain. Subcellular location: Membrane, Multi-pass membrane protein. Also known as: ATP-sensitive inward rectifier potassium channel 12, Potassium channel, inwardly rectifying subfamily J member 12, Inward rectifier K(+) channel Kir2.2, Kir2.2v, IRK2, KCNJN1.|
|Specificity||Appears to be specific for KCNJ12.|
|Application Notes||IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Wible, De Biasi, Majumder et al.: "Cloning and functional expression of an inwardly rectifying K+ channel from human atrium." in: Circulation research, Vol. 76, Issue 3, pp. 343-50, 1995 (PubMed).
Namba, Inagaki, Gonoi et al.: "Kir2.2v: a possible negative regulator of the inwardly rectifying K+ channel Kir2.2." in: FEBS letters, Vol. 386, Issue 2-3, pp. 211-4, 1996 (PubMed).
Gallagher, Forget: "An alternate promoter directs expression of a truncated, muscle-specific isoform of the human ankyrin 1 gene." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1339-48, 1998 (PubMed).
Kaibara, Ishihara, Doi et al.: "Identification of human Kir2.2 (KCNJ12) gene encoding functional inward rectifier potassium channel in both mammalian cells and Xenopus oocytes." in: FEBS letters, Vol. 531, Issue 2, pp. 250-4, 2002 (PubMed).
Dephoure, Zhou, Villén et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).
|Hosts||Rabbit (13), Mouse (6)|
|Reactivities||Human (10), Rat (Rattus) (10), Mouse (Murine) (7), Cow (Bovine) (2), Dog (Canine) (2), Cat (Feline) (1), Chicken (1)|
|Applications||Western Blotting (WB) (17), Immunohistochemistry (IHC) (10), ELISA (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunocytochemistry (ICC) (2), Immunofluorescence (IF) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2), Immunostaining (ISt) (2), Immunoprecipitation (IP) (1)|
|Epitopes||Cytoplasmic Domain (5), AA 362-427 (2), Cytoplasmic,C-Term,AA 390-410 (1), Internal Region (1)|