Nerve Growth Factor Receptor (NGFR) antibody

Antigen: Nerve Growth Factor Receptor (NGFR)

Synonyms: CD271, p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, MGC148470, NGFR, wu:fb38c02, wu:fc10h01, p75, LNGFR, RNNGFRR, nrh1, NRH1b, p75ntr, tnfrsf16, MGC131034

Clonality: Monoclonal

Host: Mouse

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN350707

Additional Information

Alternative name: p75NTR (low affinity NGF receptor, MC192)

Swiss-Prot: P07174

Immunogen: Nerve growth factor receptor (TNFR superfamily, member 16)

Format: Lyophilized

Isotype: IgG1  (Matching secondary antibodies)

Description: FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Also known as: TNR16, low-affinity nerve growth factor receptor, NGF receptor, Gp80-LNGFR, p75 ICD, low affinity neurotrophin receptor p75NTR, Tnfrsf16, Ngfr, p75, LNGFR, RNNGFRR.

Specificity: Appears to be specific for rat p75NTR.

Application Details

Application Notes: WB, IHC, ELISA. A working concentration of 5-15 µg/ml is recommended. This antibody is suitable for use in paraformaldehyde fixed, alcohol fixed and paraffin-embedded sections. The optimal working solution should be determined by the end user.

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on rat trigeminal using Mouse monoclonal antibody to rat p75NTR (ABIN350707) at a concentration of 10 µg/ml, Rabbit antibody to internal part of cFos (c-fos): whole serum (ABIN350207) at 1 : 500 dilution and DAPI counterstained.

IF on rat trigeminal using Mouse monoclonal antibody to rat p75NTR (ABIN350707) at a concentration of 10 µg/ml.

IF on rat trigeminal using Mouse monoclonal antibody to rat p75NTR (ABIN350707) at a concentration of 10 µg/ml (green), Rabbit antibody to internal part of cFos (c-fos): whole serum (ABIN350207) at 1 : 500 dilution and DAPI counterstained appearing in blue.

Publications

Publications: Johnson, Lanahan, Buck et al.: "Expression and structure of the human NGF receptor." in: Cell, Vol. 47, Issue 4, pp. 545-54, 1986 (PubMed).

Nemoto, Kageyama, Hagiwara et al.: "Mutation of low affinity nerve growth factor receptor gene in spontaneously hypertensive and stroke-prone spontaneously hypertensive rats: one of the promising candidate genes for hypertension." in: Brain research, Vol. 655, Issue 1-2, pp. 267-70, 1995 (PubMed).

Sposato, Manni, Chaldakov et al.: "Streptozotocin-induced diabetes is associated with changes in NGF levels in pancreas and brain." in: Archives italiennes de biologie, Vol. 145, Issue 2, pp. 87-97, 2007 (PubMed).

Sivilia, Paradisi, DIntino et al.: "Skin homeostasis during inflammation: a role for nerve growth factor." in: Histology and histopathology, Vol. 23, Issue 1, pp. 1-10, 2007 (PubMed).