NGFR antibody (AA 281-421)

Catalog No. ABIN6940196

The Mouse Monoclonal anti-NGFR antibody is suitable to detect NGFR in samples from Human. It has been validated for IHC.

Quick Overview for NGFR antibody (AA 281-421) (ABIN6940196)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: NGFR-1964

Product Details anti-NGFR Antibody

Binding Specificity: AA 281-421

Purpose: NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Antibody

Specificity: It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human p75 NGFR protein (around aa 281-421) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Neuronal axons, Schwann cells and perineural cells of peripheral nerves or tumors of nerve sheath differentiation e. G. Schwannoma, Neurofibroma. Soma and axons of sensory neurons and ganglionic satellite cells. Melanomas.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background:

CD271, Gp80-LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Nerve growth factor receptor (NGFR), p75 ICD, p75 Neurotrophin receptor, Tumor necrosis factor receptor superfamily member 16 (TNFRSF16)

Neurofibroma. Soma and axons of sensory neurons and ganglionic satellite cells. Melanomas., Neuronal axons, Schwann cells and perineural cells of peripheral nerves or tumors of nerve sheath differentiation e. G. Schwannoma

Molecular Weight: 75kDa

Gene ID: 4804, 415768, 681726

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding