VAMP7 antibody

Catalog No. ABIN351365

The Rabbit Polyclonal anti-VAMP7 antibody has been validated for WB and IHC. It is suitable to detect VAMP7 in samples from Rat.

Quick Overview for VAMP7 antibody (ABIN351365)

Target: VAMP7 (Vesicle-Associated Membrane Protein 7 (VAMP7))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAMP7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-VAMP7 Antibody

Purpose: Rabbit antibody to VAMP7

Specificity: Specific for VAMP7.

Cross-Reactivity: Human, Marmoset, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from rat VAMP7 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.

Isotype: IgG

Application Details

Application Notes: IHC WB. A working concentration of 10-50,micro,g,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for VAMP7

Target: VAMP7 (Vesicle-Associated Membrane Protein 7 (VAMP7))

Alternative Name: VAMP7

Background: FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. SUBUNIT: Component of the SNARE complex composed of STX4 SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7 STX8 VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells. SUBCELLULAR LOCATION: Cytoplasmic vesicle secretory vesicle membrane, Single-pass type IV membrane protein. Golgi apparatus trans-Golgi network membrane, Single-pass type IV membrane protein. Late endosome membrane, Single-pass type IV membrane protein. Lysosome membrane, Single-pass type IV membrane protein. Endoplasmic reticulum membrane, Single-pass type IV membrane protein. Cytoplasmic vesicle phagosome membrane, Single-pass type IV membrane protein. TISSUE SPECIFICITY: Detected in all tissues tested.

UniProt: Q9JHW5