CD5 antibody (Biotin)

Catalog No. ABIN356200

This Rat Monoclonal antibody specifically detects CD5 in FACS, WB, IP, IHC (fro) and Func. It exhibits reactivity toward Mouse.

Quick Overview for CD5 antibody (Biotin) (ABIN356200)

Target: CD5

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: 4H8E6

Product Details anti-CD5 Antibody (Biotin)

Specificity: This antibody is specific to CD5/Lyt-1 (Mr. 67 kDa)

Characteristics: Synonyms: Lymphocyte antigen T1/Leu-1, LEU1, T-cell surface glycoprotein CD5

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Identification and enumeration of CD5+ cells (< / = 1 μg/10^6 cells). (Ref.15)Immunoprecipitation. (Ref.15)Immunohistochemistry on Frozen Sections. (Ref.15)T Cell Activation. (Ref.15)Western blotting. (Ref.15)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (Ref.1-3) It has been identified as the major ligand of the B-cell antigen CD72. (Ref.4,5) The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in Ref.6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (Ref.7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (Ref.13,14)Synonyms: CD5, LEU1, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

Gene ID: 12507

UniProt: P13379