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Details for Product No. ABIN356280

ACTA2 / Aortic Smooth Muscle Actin (N-Term) antibody

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Antigen
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Cow (Bovine), Chicken, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 2 references available
Catalog no. ABIN356280
Quantity 1 mL
Price
335.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen N-terminal decapeptide of alpha smooth muscle isoform of actin, acetylated at theN-terminus. Remarks: Epitope: Acetyl group with N- terminal four amino acids of peptide chain of alphasmooth muscle.
Clone Aasm 204 (1A4)
Isotype IgG2a
Characteristics Synonyms: Alpha-actin-2, ACTVS, GIG46, Cell growth-inhibiting gene 46 protein
Purification Purified
Background Actin is a globular, roughly 42-kDa protein found in all eukaryotic cells. Actin participatesin many important cellular functions, including muscle contraction cell motility celldivision and cytokinesis vesicle and organelle movement, cell signaling, and theestablishment and maintenance of cell junctions and cell shape. Expression of smoothmuscle alpha actin is regulated by hormones and cell proliferation and is altered bypathological conditions including onogenic transformation and atherosclerosis.
UniProt P62736
Application Notes Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidin~biotin system orpolymer system, incubate 30 minutes at room temperature. Recommended Positive Control: Human uterus, gastrointestinal tissue, Leiomyosarcoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative.
Preservative Sodium azide
Storage -20 °C
Storage Comment Storethe antibody undiluted at 2-8Cfor one month or (in aliquots) at -20Cfor longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Miwa, Manabe, Kurokawa et al.: "Structure, chromosome location, and expression of the human smooth muscle (enteric type) gamma-actin gene: evolution of six human actin genes." in: Molecular and cellular biology, Vol. 11, Issue 6, pp. 3296-306, 1991 (PubMed).

Skalli, Ropraz, Trzeciak et al.: "A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 2, pp. 2787-96, 1987 (PubMed).

Alternatives for antigen "ACTA2 / Aortic Smooth Muscle Actin", type "Antibodies"
Hosts (18), (12), (2)
Reactivities (27), (16), (16), (13), (13), (12), (5), (4), (1), (1)
Applications (25), (18), (9), (9), (6), (2), (2)
Conjugates (1)
Epitopes (17), (1), (1)
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