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Details for Product No. ABIN358108

Checkpoint Kinase 1 (CHEK1) (pSer280) antibody

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Synonyms CHK1, Chk1, rad27, C85740, CHEK1, chk1, id:ibd2720, zgc:56093, An08g10320, AO090003000441
»Alternatives pSer280
»Alternatives Human, Mouse (Murine)
»Alternatives Rabbit
Clonality Polyclonal
»Alternatives Un-conjugated
»Alternatives ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN358108
Quantity 0.4 mL
346.50 $   Plus shipping costs $45.00
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280 region of Human CHK1. Genename: CHEK1
Specificity This antibody detects CHK1 pSer280.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name CHK1
Background Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.
Alternate names: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
Molecular Weight 54420 Da
Gene ID 1111
NCBI Accession 9606
UniProt O14757
Research Area Kinases/Phosphatases, Cell Cycle
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Groth, Lukas, Nigg et al.: "Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint." in: The EMBO journal, Vol. 22, Issue 7, pp. 1676-87, 2003 (PubMed).

Chen, Ryan, Piwnica-Worms: "Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding." in: Molecular and cellular biology, Vol. 23, Issue 21, pp. 7488-97, 2003 (PubMed).

Ng, Lee, Ho et al.: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004 (PubMed).

Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004 (PubMed).

Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts (290), (37), (11), (5)
Reactivities (332), (126), (124), (36), (26), (25), (14), (4), (3), (3), (2), (1), (1)
Applications (247), (135), (66), (65), (50), (37), (21), (19), (7), (3), (3), (2), (1)
Conjugates (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (39), (38), (31), (26), (13), (7), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)