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CHEK1 antibody (pSer280)

CHEK1 Reactivity: Human, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN358108
  • Target See all CHEK1 Antibodies
    CHEK1 (Checkpoint Kinase 1 (CHEK1))
    Binding Specificity
    • 44
    • 29
    • 26
    • 18
    • 14
    • 13
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer280
    Reactivity
    • 214
    • 118
    • 68
    • 16
    • 9
    • 9
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human, Mouse
    Host
    • 223
    • 14
    • 7
    • 5
    Rabbit
    Clonality
    • 209
    • 41
    Polyclonal
    Conjugate
    • 134
    • 13
    • 11
    • 9
    • 9
    • 9
    • 9
    • 8
    • 8
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    This CHEK1 antibody is un-conjugated
    Application
    • 172
    • 87
    • 54
    • 41
    • 40
    • 35
    • 35
    • 17
    • 16
    • 13
    • 10
    • 7
    • 5
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects CHK1 pSer280.
    Purification
    Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
    Immunogen
    This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280 region of Human CHK1. Genename: CHEK1
    Isotype
    Ig Fraction
    Top Product
    Discover our top product CHEK1 Primary Antibody
  • Application Notes
    ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS with 0.09 % (W/V) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    CHEK1 (Checkpoint Kinase 1 (CHEK1))
    Alternative Name
    CHK1 (CHEK1 Products)
    Synonyms
    An08g10320 antibody, AO090003000441 antibody, CHK1 antibody, chk1 antibody, C85740 antibody, Chk1 antibody, rad27 antibody, id:ibd2720 antibody, zgc:56093 antibody, CHEK1 antibody, checkpoint kinase 1 antibody, serine/threonine-protein kinase chk1 antibody, serine/threonine-protein kinase Chk1 antibody, CAMK/CAMKL/CHK1 protein kinase Chk1 antibody, checkpoint kinase 1 S homeolog antibody, CHEK1 antibody, ANI_1_2436074 antibody, AOR_1_768154 antibody, PTRG_04183 antibody, SJAG_01680 antibody, PAAG_04978 antibody, MCYG_03290 antibody, VDBG_03742 antibody, chek1.S antibody, Chek1 antibody, chek1 antibody
    Background
    Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
    Molecular Weight
    54420 Da
    Gene ID
    1111, 9606
    UniProt
    O14757
    Pathways
    p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair
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