Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody

Antigen: Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1)

Synonyms: HSPD1, DKFZp459H2320, HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, hld4, cpn60, groel, hsp60, hsp65, spg13, chaperonin, cb863, fa04a05, fb22d10, fi27b05, sb:cb144, id:ibd2197, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05, MIF4, MNA2, mopA, groL, crpA

Clonality: Monoclonal (LK1)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster), Guinea Pig, Hamster, Monkey, Pig (Porcine), Rabbit, Sheep (Ovine), Xenopus laevis

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS), Immunhistochemisch (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN361784

Additional Information

Alternative name: Hsp60

Gene ID: 3329

UniProt: P10809

Immunogen: Recombinant human Hsp60

Isotype: IgG1

Clone: LK1

Description: Synonyms: CPN60, GROEL, HLD4, Hsp 60, Hsp65, HSPD1, HuCHA60, SPG 13

Characteristics: Accession Number: NP_002147.2

Specificity: Detects an ~60kDa protein corresponding to the molecular mass of Hsp60 on SDS PAGE immunoblots.

Sensitivity: 0.05µg/mL of SMC-110 was sufficient for detection of Hsp60 in 20µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Synonyms: HSPD1, DKFZp459H2320, HLD4, CPN60, GROEL, HSP60, HSP65, SPG13, HuCHA60, hld4, cpn60, groel, hsp60, hsp65, spg13, chaperonin, cb863, fa04a05, fb22d10, fi27b05, sb:cb144, id:ibd2197, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05, MIF4, MNA2, mopA, groL, crpA

Application Details

Application Notes: 0.05 µg/mL (WB)

Concentration: 1mg/mL

Purification: Protein G Purified

Buffer: PBS, 50% glycerol

Storage: Store at -20° C. Shipping Conditions: Blue Ice or 4° C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Heat Shock Proteins, Signaling

Restrictions: For Research Use only

Images

Hsp60 (LK 1), cell line mix.

Publications

Jindal, Dudani, Singh et al.: "Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen." in: Molecular and cellular biology, Vol. 9, Issue 5, pp. 2279-83, 1989 (PubMed).

Gao, Brosnan, Raine: "Experimental autoimmune encephalomyelitis. Qualitative and semiquantitative differences in heat shock protein 60 expression in the central nervous system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3548-56, 1995 (PubMed).

Hartl: "Molecular chaperones in cellular protein folding." in: Nature, Vol. 381, Issue 6583, pp. 571-9, 1996 (PubMed).

Neuer, Lam, Tiller et al.: "Humoral immune response to membrane components of Chlamydia trachomatis and expression of human 60 kDa heat shock protein in follicular fluid of in-vitro fertilization patients." in: Human reproduction (Oxford, England), Vol. 12, Issue 5, pp. 925-9, 1997 (PubMed).

Bukau, Horwich: "The Hsp70 and Hsp60 chaperone machines." in: Cell, Vol. 92, Issue 3, pp. 351-66, 1998 (PubMed).

Hartl, Hayer-Hartl: "Molecular chaperones in the cytosol: from nascent chain to folded protein." in: Science (New York, N.Y.), Vol. 295, Issue 5561, pp. 1852-8, 2002 (PubMed).

Itoh, Komatsuda, Ohtani et al.: "Mammalian HSP60 is quickly sorted into the mitochondria under conditions of dehydration." in: European journal of biochemistry / FEBS, Vol. 269, Issue 23, pp. 5931-8, 2002 (PubMed).

Bason, Corrocher, Lunardi et al.: "Interaction of antibodies against cytomegalovirus with heat-shock protein 60 in pathogenesis of atherosclerosis." in: Lancet, Vol. 362, Issue 9400, pp. 1971-7, 2003 (PubMed).

Gupta, Knowlton: "HSP60, Bax, apoptosis and the heart." in: Journal of cellular and molecular medicine, Vol. 9, Issue 1, pp. 51-8, 2005 (PubMed).

Deocaris, Kaul, Wadhwa: "On the brotherhood of the mitochondrial chaperones mortalin and heat shock protein 60." in: Cell stress & chaperones, Vol. 11, Issue 2, pp. 116-28, 2006 (PubMed).

Lai, Zhuang, Zhang: "[Stability of implants placed in different bone types]" in: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology, Vol. 42, Issue 5, pp. 292-3, 2007 (PubMed).

Verda, Kim, Ikehara et al.: "Hematopoietic mixed chimerism derived from allogeneic embryonic stem cells prevents autoimmune diabetes mellitus in NOD mice." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 2, pp. 381-6, 2008 (PubMed).

Ahsan, Donnart, Nouri et al.: "Tissue-Specific Defense and Thermo-Adaptive Mechanisms of Soybean Seedlings under Heat Stress Revealed by Proteomic Approach." in: Journal of proteome research, 2010 (PubMed).