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Details for Product No. ABIN371041

Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) antibody (Biotin)

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Antigen
Synonyms APA, CD249, gp160, 6030431M22Rik, Bp-1/6C3, Ly-51, Ly51, im:7152184, si:ch211-146m5.2
Reactivity
»Alternatives Mouse (Murine)
Host
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Biotin
Application
»Alternatives Flow Cytometry (FACS)
Pubmed 9 references available
Catalog no. ABIN371041
Quantity 0.5 mg
Price
313.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Clone FG35-4
Isotype IgG1
Characteristics Synonyms: EAP, Aminopeptidase A, ENPEP, Differentiation antigen gp160
Alternative Name CD249 / Glutamyl aminopeptidase
Background BP1, also known as Ly51/6C3 antigen, is a type II homodimeric transmembraneglycoprotein formed by two disulfide-linked chains of 140 kDa. (Ref. 1,2) Originallyidentified on the surface of pre-B cell lymphomas (Ref. 1,3) the cDNA encoding BP1 hasbeen cloned and found to represent Aminopeptidase A. (Ref. 4,5) It is expressed at highlevels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on awide variety of Mouse tissues known to possess aminopeptidase activity. (Ref. 6-8)Subsets of normal bone marrow pre-B and B cells express low levels of BP1, which israpidly upregulated on pre-B cells in the presence of IL-7. (Ref. 9,10)The BP1 marker is commonly used to identify B cells in different stages of development. (Ref. 11)
UniProt P16406
Application Notes Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.12)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Wu, Lahti, Air et al.: "Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 3, pp. 993-7, 1990 (PubMed).

Hardy, Carmack, Shinton et al.: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, 1991 (PubMed).

Wu, Li, Cooper et al.: "Aminopeptidase A activity of the murine B-lymphocyte differentiation antigen BP-1/6C3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 2, pp. 676-80, 1991 (PubMed).

Welch, Burrows, Namen et al.: "Bone marrow stromal cells and interleukin-7 induce coordinate expression of the BP-1/6C3 antigen and pre-B cell growth." in: International immunology, Vol. 2, Issue 8, pp. 697-705, 1991 (PubMed).

Sherwood, Weissman: "The growth factor IL-7 induces expression of a transformation-associated antigen in normal pre-B cells." in: International immunology, Vol. 2, Issue 5, pp. 399-406, 1991 (PubMed).

Cooper, Mulvaney, Coutinho et al.: "A novel cell surface molecule on early B-lineage cells." in: Nature, Vol. 321, Issue 6070, pp. 616-8, 1986 (PubMed).

Adkins, Tidmarsh, Weissman: "Normal thymic cortical epithelial cells developmentally regulate the expression of a B-lineage transformation-associated antigen." in: Immunogenetics, Vol. 27, Issue 3, pp. 180-6, 1988 (PubMed).

Whitlock, Tidmarsh, Muller-Sieburg et al.: "Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule." in: Cell, Vol. 48, Issue 6, pp. 1009-21, 1987 (PubMed).

Li, Wu, Wang et al.: "Widespread tissue distribution of aminopeptidase A, an evolutionarily conserved ectoenzyme recognized by the BP-1 antibody." in: Tissue antigens, Vol. 42, Issue 5, pp. 488-96, 1994 (PubMed).

Alternatives for antigen "Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP)", type "Antibodies"
Hosts (28), (19), (11), (9)
Reactivities (38), (36), (7), (5), (5), (1), (1)
Applications (40), (36), (14), (14), (14), (10), (9), (2)
Conjugates (8), (7), (7), (1)
Epitopes (8), (5), (2), (2), (1), (1)
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