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Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) antibody (Biotin)

Details for Product No. ABIN371041, Supplier: Login to see
Antigen
  • im:7152184
  • si:ch211-146m5.2
  • 6030431M22Rik
  • APA
  • Bp-1/6C3
  • Ly-51
  • Ly51
  • CD249
  • gp160
Reactivity
Mouse (Murine)
100
28
13
9
5
5
3
1
1
Host
Rat
65
22
20
20
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
13
12
8
8
3
3
2
Application
Flow Cytometry (FACS)
85
53
53
45
19
15
9
3
1
1
1
1
1
1
1
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Clone FG35-4
Isotype IgG1
Specificity This antibody is specific to BP1 (Ly-51/6C3).
Characteristics Synonyms: EAP, Aminopeptidase A, ENPEP, Differentiation antigen gp160
Alternative Name CD249 / Glutamyl Aminopeptidase (ENPEP Antibody Abstract)
Background BP1, also known as Ly51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide-linked chains of 140 kDa. (Ref.1,2) Originally identified on the surface of pre-B cell lymphomas (Ref.1,3) the cDNA encoding BP1 has been cloned and found to represent Aminopeptidase A. (Ref.4,5) It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of Mouse tissues known to possess aminopeptidase activity. (Ref.6-8) Subsets of normal bone marrow pre-B and B cells express low levels of BP1, which is rapidly upregulated on pre-B cells in the presence of IL-7. (Ref.9,10) The BP1 marker is commonly used to identify B cells in different stages of development. (Ref.11)Synonyms: Aminopeptidase A, Differentiation antigen gp160, EAP, ENPEP
Gene ID 13809
UniProt P16406
Pathways
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.12)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Li, Wu, Wang et al.: "Widespread tissue distribution of aminopeptidase A, an evolutionarily conserved ectoenzyme recognized by the BP-1 antibody." in: Tissue antigens, Vol. 42, Issue 5, pp. 488-96, 1994 (PubMed).

Whitlock, Tidmarsh, Muller-Sieburg et al.: "Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule." in: Cell, Vol. 48, Issue 6, pp. 1009-21, 1987 (PubMed).

Adkins, Tidmarsh, Weissman: "Normal thymic cortical epithelial cells developmentally regulate the expression of a B-lineage transformation-associated antigen." in: Immunogenetics, Vol. 27, Issue 3, pp. 180-6, 1988 (PubMed).

Cooper, Mulvaney, Coutinho et al.: "A novel cell surface molecule on early B-lineage cells." in: Nature, Vol. 321, Issue 6070, pp. 616-8, 1986 (PubMed).

Sherwood, Weissman: "The growth factor IL-7 induces expression of a transformation-associated antigen in normal pre-B cells." in: International immunology, Vol. 2, Issue 5, pp. 399-406, 1991 (PubMed).

Welch, Burrows, Namen et al.: "Bone marrow stromal cells and interleukin-7 induce coordinate expression of the BP-1/6C3 antigen and pre-B cell growth." in: International immunology, Vol. 2, Issue 8, pp. 697-705, 1991 (PubMed).

Wu, Li, Cooper et al.: "Aminopeptidase A activity of the murine B-lymphocyte differentiation antigen BP-1/6C3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 2, pp. 676-80, 1991 (PubMed).

Hardy, Carmack, Shinton et al.: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, 1991 (PubMed).

Wu, Lahti, Air et al.: "Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 3, pp. 993-7, 1990 (PubMed).