Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
CD1 Molecules, like MHC I and II, play an equally important role in the immune system by presenting lipid, glycolipid and lipopeptide antigen to T and NKT cells. (Ref.1) Chicken CD1 gene is located in the Chicken MHC B locus and has an important implication for the primordial MHC gene evolution. (Ref.2) Two proteins have been identified: CD1.1 and CD1.2. Analysis of RNA from blood cells showed that both genes are expressed in Bu-1+ cells (B cells and some macrophages) and CD8a+ cells (some T and NK cells). CD1.2 is expressed in TCR1 + cells (gamma delta cells) but is nearly undetectable in TCR2+ and TCR3+ cells (alpha and Beta T cells). (Ref.1)Synonyms: T cell surface glycoprotein CD1.1