B-Cell CLL/lymphoma 2 (BCL2) (AA 61-76) antibody

Details for Product No. ABIN371613
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms AW986256, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik, PPP1R50, BCL-2, bcl-2, Bcl2
Epitope
AA 61-76
(52), (44), (37), (28), (26), (22), (20), (13), (13), (12), (9), (8), (6), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus)
(455), (159), (125), (54), (36), (36), (14), (12), (3), (2), (2)
Host
Mouse
(373), (125), (9), (7), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(22), (17), (14), (13), (9), (7), (7), (7), (7), (7), (7), (7), (7), (6), (1), (1)
Application
ELISA, Western Blotting (WB)
(339), (180), (141), (123), (90), (87), (70), (58), (56), (43), (24), (10), (5), (2), (2), (2), (2), (1), (1)
Pubmed 12 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN371613
286.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen KLH-conjugated peptide corresponding to 61-76 amino acid sequence of murine Bcl-2. (Ref. 1)
Clone 10C2
Isotype IgG1
Characteristics Synonyms: BCL2, Bcl-2 alpha, Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2
Purification Purified
Alternative Name Bcl-2
Background Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellularsystems. (Ref. 2) It plays a key role in tissue and organ development as well as in adulttissues during cell turnover. Apoptosis can be induced by a variety of internal and externalstimuli including growth factor deprivation, cytokine treatment, antigen-receptorengagement, cell-cell interactions, irradiation and glucocorticoid treatment. (Ref. 3)Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor ofprogrammed cell death. It has been localized to the outer mitochondrial membrane,perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory andresting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes,and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli,including growth factor deprivation, gamma-irradiation, glucocorticoids, andchemotherapeutic agents. During lymphoid development, expression of the Bcl-2 proteinappears to be regulated in a stage-specific manner, and is thought to be a survival signalfor positive selection.
UniProt P10417
Research Area Cancer, Apoptosis/Necrosis
Application Notes ELISA. Western Blot: < / = 1 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.2 without preservatives or amine-containing buffer salts.
Preservative Without preservative
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Cohen: "Programmed cell death in the immune system." in: Advances in immunology, Vol. 50, pp. 55-85, 1991 (PubMed).

Cory: "Regulation of lymphocyte survival by the bcl-2 gene family." in: Annual review of immunology, Vol. 13, pp. 513-43, 1995 (PubMed).

Farrow, White, Martinou et al.: "Cloning of a bcl-2 homologue by interaction with adenovirus E1B 19K." in: Nature, Vol. 374, Issue 6524, pp. 731-3, 1995 (PubMed).

Chittenden, Harrington, OConnor et al.: "Induction of apoptosis by the Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 733-6, 1995 (PubMed).

Kiefer, Brauer, Powers et al.: "Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak." in: Nature, Vol. 374, Issue 6524, pp. 736-9, 1995 (PubMed).

Shimizu, Eguchi, Kosaka et al.: "Prevention of hypoxia-induced cell death by Bcl-2 and Bcl-xL." in: Nature, Vol. 374, Issue 6525, pp. 811-3, 1995 (PubMed).

Yin, Oltvai, Korsmeyer: "BH1 and BH2 domains of Bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax." in: Nature, Vol. 369, Issue 6478, pp. 321-3, 1994 (PubMed).

Oltvai, Milliman, Korsmeyer: "Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death." in: Cell, Vol. 74, Issue 4, pp. 609-19, 1993 (PubMed).

Cheng, Levine, Boise et al.: "Bax-independent inhibition of apoptosis by Bcl-XL." in: Nature, Vol. 379, Issue 6565, pp. 554-6, 1996 (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed).

Nuñez, Clarke: "The Bcl-2 family of proteins: regulators of cell death and survival." in: Trends in cell biology, Vol. 4, Issue 11, pp. 399-403, 2004 (PubMed).

Gottschalk, Boise, Oltvai et al.: "The ability of Bcl-x(L) and Bcl-2 to prevent apoptosis can be differentially regulated." in: Cell death and differentiation, Vol. 3, Issue 1, pp. 113-8, 2006 (PubMed).

Validation Images
Did you look for something else?
back to top