Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pSer1480) antibody

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Antigen
Synonyms AW490526, GluN2B, NR2B, Nmdar2b, MRD6, NMDAR2B, hNR3, nr2b, nmdar2b
Epitope
pSer1480
(42), (38), (35), (26), (22), (21), (17), (16), (7), (6), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(289), (277), (256), (138), (135), (134), (70), (17), (8), (7), (4), (3), (3), (2), (1)
Host
Rabbit
(298), (24), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (6), (6), (6), (6), (6), (1), (1)
Application
Western Blotting (WB)
(194), (96), (90), (90), (44), (41), (37), (20), (19), (11), (4), (2), (1)
Pubmed 7 references available
Catalog no. ABIN372688
Quantity 0.1 mL
Price
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser1480 of the NR2B subunit of the Rat NMDA Receptor.
Isotype IgG
Specificity This antibody recognizes the ~180k NMDAR NR2B subunit protein phosphorylated at Ser1480. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by lambda-phosphatase treatment.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name NMDAR2B
Background The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). It has been shown that phosphorylation of Ser1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004).
Alternate names: GRIN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA Receptor 2B
Gene ID 2904
NCBI Accession NP_000825.2
UniProt Q13224
Research Area Neurology, Neurotransmitters, Receptors
Application Notes Western blot: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20°C.
Expiry Date 12 months
Supplier Images
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pSer1480) antibody anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (pSer1480) antibody
Background publications Ishii, Moriyoshi, Sugihara et al.: "Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits." in: The Journal of biological chemistry, Vol. 268, Issue 4, pp. 2836-43, 1993 (PubMed).

Grosshans, Clayton, Coultrap et al.: "LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1." in: Nature neuroscience, Vol. 5, Issue 1, pp. 27-33, 2001 (PubMed).

Takasu, Dalva, Zigmond et al.: "Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors." in: Science (New York, N.Y.), Vol. 295, Issue 5554, pp. 491-5, 2002 (PubMed).

Carroll, Zukin: "NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity." in: Trends in neurosciences, Vol. 25, Issue 11, pp. 571-7, 2002 (PubMed).

Wenthold, Prybylowski, Standley et al.: "Trafficking of NMDA receptors." in: Annual review of pharmacology and toxicology, Vol. 43, pp. 335-58, 2003 (PubMed).

Chung, Huang, Lau et al.: "Regulation of the NMDA receptor complex and trafficking by activity-dependent phosphorylation of the NR2B subunit PDZ ligand." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 45, pp. 10248-59, 2004 (PubMed).

Baya, Maresca, Poli et al.: "Dihydrogen to dihydride isomerization mechanism in [(C5Me5)FeH2(Ph2PCH2CH2PPh2)]+ through the experimental and theoretical analysis of kinetic isotope effects." in: Inorganic chemistry, Vol. 45, Issue 25, pp. 10248-62, 2006 (PubMed).

Hosts (298), (24), (5)
Reactivities (289), (277), (256), (138), (135), (134), (70), (17), (8), (7), (4), (3), (3), (2), (1)
Applications (194), (96), (90), (90), (44), (41), (37), (20), (19), (11), (4), (2), (1)
Conjugates (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (6), (6), (6), (6), (6), (1), (1)
Epitopes (42), (38), (35), (26), (22), (21), (17), (16), (7), (6), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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