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Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (N-Term) antibody

Details for Product No. ABIN374443, Supplier: Log in to see
Antigen
  • AI323664
  • BB161688
  • SLP-76
  • SLP76
  • twm
  • Slp76
Alternatives
anti-Human Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
N-Term
26
17
15
6
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
126
55
41
9
3
2
1
1
1
Host
Goat
74
38
19
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
102
37
28
20
15
12
7
6
3
2
2
1
1
Supplier
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Immunogen Peptide from the N Terminus of the protein sequence according to NP_005556.
Sequence C-ALRNVPFRSE V
Specificity This antibody recognizes LCP2.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine, Pig, Cow.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Alternative Name LCP2 (LCP2 Antibody Abstract)
Background LCP2 is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.Synonyms: LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76
Gene ID 3937
NCBI Accession NP_005556
UniProt Q13094
Research Area Signaling
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes Peptide ELISA: 1/32,000. Western Blot: 0.5-2 μg/mL. Detects a band of Approx 75 kDa (Predicted Molecular Weight65 kDa). Positive Control: Jurkat lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.
Supplier Images
 image for anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (N-Term) antibody (ABIN374443) anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (N-Term) antibody
Background publications Jackman, Motto, Sun et al.: "Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells." in: The Journal of biological chemistry, Vol. 270, Issue 13, pp. 7029-32, 1995 (PubMed).