LCP2 antibody (AA 216-434)

Catalog No. ABIN94501

This Rabbit Polyclonal antibody specifically detects LCP2 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for LCP2 antibody (AA 216-434) (ABIN94501)

Target: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LCP2 Antibody

Binding Specificity: AA 216-434

Purpose: Anti-SLP76 Purified

Cross-Reactivity (Details): Other not tested, Human

Characteristics: The polyclonal antibody reacts with SLP76, a 76 kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils, in B cells, however, it is replaced by SLP65.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag

Application Details

Application Notes: Western blotting: Positive control: JURKAT human T cell leukemia cell lysate, reducing conditions, Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: thymus.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for LCP2

Target: LCP2 (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2))

Alternative Name: SLP76

Background: Lymphocyte cytosolic protein 2,SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets, B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.,SLP-76, LCP2

Gene ID: 3937

UniProt: Q13094

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway