LCP2 antibody (Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa)) (AA 216-434)

Details for Product anti-LCP2 Antibody No. ABIN94466
Antigen
Epitope
AA 216-434
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Reactivity
Human, Pig (Porcine), Mouse (Murine)
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90
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10
Host
Mouse
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23
7
Clonality (Clone)
Monoclonal ()
Conjugate
This LCP2 antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
Immunogen Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag
Clone SLP-76-03
Isotype IgG2b
Specificity The polyclonal antibody reacts with SLP76, a 76 kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils, in B cells, however, it is replaced by SLP65.
Cross-Reactivity (Details) Mouse, Human, Porcine
Purification Purified by protein-G affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name SLP76 (LCP2 Antibody Abstract)
Background SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets, B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (AA 216-434) antibody (ABIN94466) Western Blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76...